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songsong000

铁虫 (初入文坛)

[求助] 流式检测

在流式检测过程之前的细胞的提取部分,都有哪些因素会影响抗体与抗原的结核性,哪些步骤可以让结果更好些?在检测过程中,流式仪的操作,那些需要注意??
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robustli

银虫 (小有名气)

【答案】应助回帖

感谢参与,应助指数 +1
It may depend on what kind of cells you are using, if you are using cell line, you may not meet many issues. If you are using cells from tissues. Be sure to get some pure cell population by using some isolation kit or reagents. During staining, be sure to optimize your primary antibody staining condition such ad dilution and time. Wash appropriate times. During flow, dilute your sample to optimal concentration, and choose optimal flow speed.
immunologyparasite
2楼2013-07-16 01:09:55
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小雨莎莎

木虫 (正式写手)

【答案】应助回帖

感谢参与,应助指数 +1
细胞提取部分?指的是细胞裂解液么?影响抗体跟抗原结合有很多因素,比如说你的细胞是活细胞,还是固定过的?你的细胞是培养基里重悬还是在缓冲液里?缓冲液里重悬细胞是否对细胞造成了影响?至于流式的操作,这个真的很难说的,结果的好坏跟你设门以及对照的选择有很重要的关系的哦~还有选用的荧光素在激光器的激发波长下发光效率是否高?这都是实验前要详细查询和咨询的呢~~
3楼2013-07-18 15:29:30
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