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莘莘小白蚁

新虫 (小有名气)

[求助] 十万火急!!!求助小鼠脾细胞增殖实验

各位大虾!我培养BALB/c小鼠脾细胞为什么老是出现问题,养不活。
       想问下取脾脏细胞培养过程中,哪些步骤较关键易出问题?
       有时间限制?要冰浴操作?一般Hank's液离心洗几次,还是干脆加完全培养基洗细胞?
       另外含酚红的RPMI1640培养基和无酚红的有区别吗?脾细胞生长周期是多长时间?你们一般铺96板的密度是多大?
       本人菜鸟,恳请指点!

[ Last edited by 莘莘小白蚁 on 2013-7-4 at 11:35 ]
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