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zhouni923219

铜虫 (正式写手)

[求助] 毕赤酵母表达 β-葡萄糖苷酶,阳性克隆的筛选????

本人将要做毕赤酵母 表达 β-葡萄糖苷酶,看了很多文献的阳性克隆的筛选方法,但不知道哪种更好一些,筛到酶活高的可能性大些?????
有做过这方面的师兄师姐可以给点建议吗???
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qiuqiushine

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我也在纠结怎么筛选,PCR筛选时酵母怎么破壁,直接煮沸吗?我转化后能够在MD上长出菌落,可是做菌落PCR筛选不到阳性克隆,请教你转化后菌落PCR怎么做?谢谢
2楼2013-12-16 16:07:50
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