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不同性别家蚕【bombyx mori】34℃高温处理后的基因表达差异分析【Serial Analysis of Gene Expression（SAGE）】 为了研究家蚕在34℃环境下耐受高温的分子作用机制,我们构建了以抗高温家蚕7532品种5龄幼虫高温处理前后中肠、丝腺和脂肪体（混合）组织为材料的四个SGAE文库【Serial Analysis of Gene Expression（SAGE）】(常温（26℃）雌、雄，高温（34℃）雌、雄)。结果在获得的4个SAGE文库中Distinct Tag number为45972、56200、49467、46819，其中能够比对到基因的分别占到41.10%、35.00%、35.98%、33.16%，获得了丰富的数据。高温处理后雌、雄家蚕的基因表达差异较大，雌蚕高温后有193个基因表现上调，507个基因下调，雄蚕高温后上调、下调基因数分别为516、318，另外有许多基因在高温后表现出特异表达或沉默。差异基因的GO分析（Gene ontology）发现雌、雄蚕高温处理后其功能分布基本一致，而在synapse part、virion part、metallochaperone、cell killing、immune system process、rhythmic process显示出显著差异（p<0.05）。KEGG （Kyoto Encyclopedia of Genes and Genomes ） pathway分析显示主要的差异途径及差异基因个数为Oxidative phosphorylation：16个，Purine metabolism：13个，Pyrimidine metabolism：9个，Ubiquitin mediated proteolysis：9个，Aminoacyl-tRNA biosynthesis：8个，Glycolysis / Gluconeogenesis：8个，Arginine and proline metabolism 8个，Ribosome 8个，虽然pathway路径分析显示雌雄差异相同，但是每个途径中参与的基因种类、基因个数及其上下调模式均表现出较大差异。结果表明，高温导致家蚕基因表达水平上的明显差异，而这些差异基因也显示出了性别差异，这些数据可以为研究不同性别家蚕对高温耐受的分子机理提供研究基础。 |
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sjharry
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2楼2013-05-14 08:20:18
3楼2013-05-14 09:09:12
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4楼2013-05-14 11:25:58
【答案】应助回帖
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591508522: 金币+35, 翻译EPI+1, ★★★很有帮助, 不管怎么样 先感谢你 金币都给你 我再看看你的翻译 非常感谢 2013-05-14 16:05:37
591508522: 金币+35, 翻译EPI+1, ★★★很有帮助, 不管怎么样 先感谢你 金币都给你 我再看看你的翻译 非常感谢 2013-05-14 16:05:37
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An Serial Analysis of Gene Expression（SAGE）of Bombyx Moris with Different Sex in 34℃ To study the molecular mechanisms of temperature-resistance of bombyx mori in 34℃, this experiment has used the middle intestines, silk glands and fat bodies of the 5th instar larvae of Temperature-resistance Bombyx Mori Specie No. 7532 before and after heat treatment as materials to construct four Serial Analysis of Gene Expression（SAGE）libraries, namely, male and female in normal temperature of 26℃, male and female in high temperature of 34℃.The results indicate that the Distinct Tag Numbers of the four SAGE libraries are 45972、56200、49467、46819 respectively, and the rates of mapping to genes are 41.10%、35.00%、35.98%、33.16% in the four libraires accordingly. Abundant data are obtained through the experiment.There are large differences between the generic expressions of male and female Bombyx Moris after heat treatment. 193 genes of female Bombyx Mori are up-regulated and 507 genes are down-regulated; while the up-regulated number and down-regulated number of genes of male Bombyx Mori are 516 and 318 respectively. Additionally, plenty of genes are expressed specifically or being silenced after heat treatment.The gene ontology (GO) analysis of differential genes claims that the functional distributions of male and female Bombyx Mori after heat treatment are roughly identical, however, significant differences（p<0.05） are showed in synapse part, virion part, metallochaperone, cell killing, immune system process and rhythmic process. The KEGG （Kyoto Encyclopedia of Genes and Genomes ） Pathway Analysis reveals the major distinct ways and the number of distinct genes: Oxidative phosphorylatio: 16, Purine metabolism:13, Pyrimidine metabolism: 9, Ubiquitin mediated proteolysis: 9, Aminoacyl-tRNA biosynthesis: 8, Glycolysis / Gluconeogenesis: 8, Arginine and proline metabolism: 8, Ribosome: 8. Although the distinctions of male and female bombyx moris are shown to be similar in the pathway analysis, the sorts, numbers and up/down-regulated models of genes in every single way vary considerably. The results show that high temperature has caused significant differences in generic expression levels of bombyx mori , and those distinct genes reveals gender differences. These data can offer research foundation for studying the molecular mechanisms of temperature-resistance of bombyx mori with different sex. 你这个太专业了，我试着译了一下，你参考下吧 |
5楼2013-05-14 14:29:06