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【答案】应助回帖
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591508522: 金币+35, 翻译EPI+1, ★★★很有帮助, 不管怎么样 先感谢你 金币都给你 我再看看你的翻译 非常感谢 2013-05-14 16:05:37
An Serial Analysis of Gene Expression（SAGE）of Bombyx Moris with Different Sex in 34℃
To study the molecular mechanisms of temperature-resistance of bombyx mori in 34℃, this experiment has used the middle intestines, silk glands and fat bodies of the 5th instar larvae of Temperature-resistance Bombyx Mori Specie No. 7532 before and after heat treatment as materials to construct four Serial Analysis of Gene Expression（SAGE）libraries, namely, male and female in normal temperature of 26℃, male and female in high temperature of 34℃.The results indicate that the Distinct Tag Numbers of the four SAGE libraries are 45972、56200、49467、46819 respectively, and the rates of mapping to genes are 41.10%、35.00%、35.98%、33.16% in the four libraires accordingly. Abundant data are obtained through the experiment.There are large differences between the generic expressions of male and female Bombyx Moris after heat treatment. 193 genes of female Bombyx Mori are up-regulated and 507 genes are down-regulated; while the up-regulated number and down-regulated number of genes of male Bombyx Mori are 516 and 318 respectively. Additionally, plenty of genes are expressed specifically or being silenced after heat treatment.The gene ontology (GO) analysis of differential genes claims that the functional distributions of male and female Bombyx Mori after heat treatment are roughly identical, however, significant differences（p<0.05） are showed in synapse part, virion part, metallochaperone, cell killing, immune system process and rhythmic process. The KEGG （Kyoto Encyclopedia of Genes and Genomes ） Pathway Analysis reveals the major distinct ways and the number of distinct genes: Oxidative phosphorylatio: 16, Purine metabolism:13, Pyrimidine metabolism: 9, Ubiquitin mediated proteolysis: 9, Aminoacyl-tRNA biosynthesis: 8, Glycolysis / Gluconeogenesis: 8, Arginine and proline metabolism: 8, Ribosome: 8. Although the distinctions of male and female bombyx moris are shown to be similar in the pathway analysis, the sorts, numbers and up/down-regulated models of genes in every single way vary considerably. The results show that high temperature has caused significant differences in generic expression levels of bombyx mori , and those distinct genes reveals gender differences. These data can offer research foundation for studying the molecular mechanisms of temperature-resistance of bombyx mori with different sex.
你这个太专业了，我试着译了一下，你参考下吧 |
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