| 查看: 694 | 回复: 5 | ||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | ||
金狮水友至尊木虫 (著名写手)
|
[求助]
英译中---生物传感器论文--求科学的翻译
|
|
|
Apart from its high sensitivity, as compared to other electrochemical miRNA biosensors, the much-simplified label- free approach makes the proposed biosensor particularly attractive for routine miRNA analysis. 30,31 To date, all miRNA assays must work with highly purified RNA samples. The freedom from chemical/biological ligation and PCR amplification greatly improves the suitability in direct profiling miRNAs with minimal or no sample pretreatment. Attempts were thus made in utilizing the biosensor for analyzing circulating miRNAs in blood and miRNAs in total RNA extract from culture cells. The same sample (except serum) was simultaneously analyzed by the biosensor and by qPCR. As presented in Table 1, good agreements between the results obtained by the biosensor and qPCR on the same sample confirmed the practical value of the biosensor. In addition, the results obtained were also consistent with published data. 32,33 Because of its high sensitivity, the amount of RNA needed for asuccessful miRNA detection was in the range of 3−10 ng. In addition, a direct analysis of let-7 miRNA in serum was attempted. Unfortunately, persistently lower values with much larger variations (30%−50%) were obtained. The results were even worse when RNA extraction efficiency is taken into consideration. Evidently, the high protein content and other blood constituents in serum severely interfere with the detection of miRNA, probably by fouling the biosensor through nonspecific adsorption. Efforts in developing an antifouling interface while maintaining most of the attractive features of the biosensor are underway. At the present time, this work only serves as a proof-of- concept through rather labor-intensive manual operation. Nonetheless, as with other electronic/electrochemical bio- sensors, both low- and high-density sensor arrays can, in principle, be constructed by adopting the standard micro- fabrication technology and the complementary metal-oxide semiconductor technology that require no additional processes other than a simple switching mechanism. Two prominent examples demonstrating the power and capacity of the electronic/electrochemical detection systems are the electro- chemical microarray developed by combimatrix 34 and the Ion Proton Sequencer recently developed by Life Technologies. 35 For example, up to 12 million individal sensors can be fabricated on a single chip in the Ion Proton Sequencer. Compared to other label-free electrochemical biosensors, 12−15 the much simplified procedure implies that the throughput can also be significantly upscaled by upgrading the manual operation to a microfluidic cartridge. Of course, similar to all other hybridization-based systems, great care must be taken when profiling multiple miRNAs sinutaneously, since there is very little room to fine-tune the hybridization conditions for all miRNAs. |
回复此楼» 猜你喜欢
求调剂
已经有23人回复
-
301求调剂
已经有15人回复
-
304求调剂（085602，过四级，一志愿985）
已经有17人回复
-
302分求调剂 一志愿安徽大学085601
已经有12人回复
-
288环境专硕,求调材料方向
已经有23人回复
-
环境专硕调剂
已经有6人回复
-
22408 调剂材料
已经有6人回复
-
285求调剂
已经有12人回复
-
求调剂
已经有6人回复
-
085600材料与化工301分求调剂院校
已经有19人回复
金狮水友
至尊木虫 (著名写手)
- 应助: 9 (幼儿园)
- 金币: 12278.2
- 散金: 50
- 红花: 13
- 沙发: 5
- 帖子: 1686
- 在线: 203小时
- 虫号: 1817809
- 注册: 2012-05-15
- 性别: GG
- 专业: 功能与智能高分子
4楼2013-05-11 13:11:49
yuankanxue
金虫 (著名写手)
分子玩家
- 应助: 38 (小学生)
- 金币: 1006.8
- 散金: 98
- 红花: 2
- 帖子: 1692
- 在线: 427.1小时
- 虫号: 595695
- 注册: 2008-09-08
- 性别: GG
- 专业: 波谱分析与成像分析
2楼2013-05-11 10:18:00
金狮水友
至尊木虫 (著名写手)
- 应助: 9 (幼儿园)
- 金币: 12278.2
- 散金: 50
- 红花: 13
- 沙发: 5
- 帖子: 1686
- 在线: 203小时
- 虫号: 1817809
- 注册: 2012-05-15
- 性别: GG
- 专业: 功能与智能高分子
3楼2013-05-11 12:28:03
yuankanxue
金虫 (著名写手)
分子玩家
- 应助: 38 (小学生)
- 金币: 1006.8
- 散金: 98
- 红花: 2
- 帖子: 1692
- 在线: 427.1小时
- 虫号: 595695
- 注册: 2008-09-08
- 性别: GG
- 专业: 波谱分析与成像分析
5楼2013-05-11 14:54:26
