24小时热门版块排行榜    

北京石油化工学院2026年研究生招生接收调剂公告
查看: 693  |  回复: 5
当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖

金狮水友

至尊木虫 (著名写手)

[求助] 英译中---生物传感器论文--求科学的翻译

Apart from its high sensitivity, as compared to other
electrochemical miRNA biosensors, the much-simplified label-
free approach makes the proposed biosensor particularly
attractive for routine miRNA analysis.
30,31
To date, all
miRNA assays must work with highly purified RNA samples.
The freedom from chemical/biological ligation and PCR
amplification greatly improves the suitability in direct profiling
miRNAs with minimal or no sample pretreatment. Attempts
were thus made in utilizing the biosensor for analyzing
circulating miRNAs in blood and miRNAs in total RNA extract
from culture cells. The same sample (except serum) was
simultaneously analyzed by the biosensor and by qPCR. As
presented in Table 1, good agreements between the results
obtained by the biosensor and qPCR on the same sample
confirmed the practical value of the biosensor. In addition, the
results obtained were also consistent with published data.
32,33
Because of its high sensitivity, the amount of RNA needed for asuccessful miRNA detection was in the range of 3−10 ng. In
addition, a direct analysis of let-7 miRNA in serum was
attempted. Unfortunately, persistently lower values with much
larger variations (30%−50%) were obtained. The results were
even worse when RNA extraction efficiency is taken into
consideration. Evidently, the high protein content and other
blood constituents in serum severely interfere with the
detection of miRNA, probably by fouling the biosensor through
nonspecific adsorption. Efforts in developing an antifouling
interface while maintaining most of the attractive features of the
biosensor are underway.
At the present time, this work only serves as a proof-of-
concept through rather labor-intensive manual operation.
Nonetheless, as with other electronic/electrochemical bio-
sensors, both low- and high-density sensor arrays can, in
principle, be constructed by adopting the standard micro-
fabrication technology and the complementary metal-oxide
semiconductor technology that require no additional processes
other than a simple switching mechanism. Two prominent
examples demonstrating the power and capacity of the
electronic/electrochemical detection systems are the electro-
chemical microarray developed by combimatrix
34
and the Ion
Proton Sequencer recently developed by Life Technologies.
35
For example, up to 12 million individal sensors can be
fabricated on a single chip in the Ion Proton Sequencer.
Compared to other label-free electrochemical biosensors,
12−15
the much simplified procedure implies that the throughput can
also be significantly upscaled by upgrading the manual
operation to a microfluidic cartridge. Of course, similar to all
other hybridization-based systems, great care must be taken
when profiling multiple miRNAs sinutaneously, since there is
very little room to fine-tune the hybridization conditions for all
miRNAs.

» 猜你喜欢

读书、读人、读世界。
已阅   关注TA 给TA发消息 送TA红花 TA的回帖

金狮水友

至尊木虫 (著名写手)

希望有人全部翻译啊
读书、读人、读世界。
4楼2013-05-11 13:11:49
已阅   关注TA 给TA发消息 送TA红花 TA的回帖
查看全部 6 个回答

yuankanxue

金虫 (著名写手)

分子玩家

【答案】应助回帖

太长了,请你指出你拿不准的部分。
有志者事竟成,梦想给我方向,方法是效率,努力是捷径,失败是老师。
2楼2013-05-11 10:18:00
已阅   关注TA 给TA发消息 送TA红花 TA的回帖

金狮水友

至尊木虫 (著名写手)

引用回帖:
2楼: Originally posted by yuankanxue at 2013-05-11 10:18:00
太长了,请你指出你拿不准的部分。

我希望得到全部的翻译的啊
读书、读人、读世界。
3楼2013-05-11 12:28:03
已阅   关注TA 给TA发消息 送TA红花 TA的回帖

yuankanxue

金虫 (著名写手)

分子玩家

引用回帖:
3楼: Originally posted by 金狮水友 at 2013-05-11 12:28:03
我希望得到全部的翻译的啊...

那你自己啥也不干,太懒了吧!
有志者事竟成,梦想给我方向,方法是效率,努力是捷径,失败是老师。
5楼2013-05-11 14:54:26
已阅   关注TA 给TA发消息 送TA红花 TA的回帖
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 301求调剂 +15 121. 2026-04-04 15/750 2026-04-07 02:14 by BruceLiu320
[考研] 312求调剂 +13 gtw1 2026-04-06 15/750 2026-04-06 20:25 by lijunpoly
[考研] 269电子信息求调剂,可转专业 +5 独酌wl 2026-04-06 5/250 2026-04-06 17:23 by 土木硕士招生
[考研] 319求调剂 +3 handrui 2026-04-05 3/150 2026-04-06 09:33 by jp9609
[考研] 一志愿武汉理工大学-085601材料工程(专硕)-总分353求调剂 +3 2626262626li 2026-04-02 3/150 2026-04-06 09:08 by 无际的草原
[考研] 085600,320分求调剂 +7 大馋小子 2026-04-01 8/400 2026-04-05 21:19 by 学员8dgXkO
[考研] 348求调剂 +3 车厘子zzz 2026-04-05 3/150 2026-04-05 20:30 by 啵啵啵0119
[考研] 本科211,293分请求调剂 +8 莲菜就是藕吧 2026-04-03 9/450 2026-04-05 19:12 by 蓝云思雨
[考研] 301求调剂 +3 XYPLR 2026-04-05 4/200 2026-04-05 19:07 by XYPLR
[考研] 326求调剂 +3 顾若浮生 2026-04-05 3/150 2026-04-05 18:32 by 蓝云思雨
[考研] 081200-11408-276学硕求调剂 +4 崔wj 2026-04-04 5/250 2026-04-05 14:06 by imissbao
[考研] 341求调剂 +3 学无止境,冲 2026-04-05 3/150 2026-04-05 09:40 by lbsjt
[考研] 材料调剂 +18 一样YWY 2026-04-02 19/950 2026-04-04 22:14 by hemengdong
[考研] 309求调剂 +6 刘刘刘1231 2026-04-02 7/350 2026-04-04 13:41 by liucky
[考研] 复试调剂 +3 bvzz 2026-04-01 3/150 2026-04-03 09:47 by 蓝云思雨
[考研] 312 化工或制药调剂 +8 小小墨123 2026-04-02 9/450 2026-04-03 09:12 by zhouxiaoyu
[考研] 296求调剂 +4 sdhu 2026-04-02 4/200 2026-04-02 21:29 by baoball
[考研] 材料专业求调剂 +10 月月鸟木 2026-04-01 10/500 2026-04-02 12:57 by wxiongid
[考研] 江苏科技大学招材料研究生 +4 Su032713. 2026-04-01 5/250 2026-04-01 22:03 by cccchenso
[考研] 0710生物学求调剂! +6 叙述文 2026-03-31 6/300 2026-04-01 09:39 by JourneyLucky
信息提示
请填处理意见