| 查看: 707 | 回复: 5 | |||
金狮水友至尊木虫 (著名写手)
|
[求助]
英译中---生物传感器论文--求科学的翻译
|
|
Apart from its high sensitivity, as compared to other electrochemical miRNA biosensors, the much-simplified label- free approach makes the proposed biosensor particularly attractive for routine miRNA analysis. 30,31 To date, all miRNA assays must work with highly purified RNA samples. The freedom from chemical/biological ligation and PCR amplification greatly improves the suitability in direct profiling miRNAs with minimal or no sample pretreatment. Attempts were thus made in utilizing the biosensor for analyzing circulating miRNAs in blood and miRNAs in total RNA extract from culture cells. The same sample (except serum) was simultaneously analyzed by the biosensor and by qPCR. As presented in Table 1, good agreements between the results obtained by the biosensor and qPCR on the same sample confirmed the practical value of the biosensor. In addition, the results obtained were also consistent with published data. 32,33 Because of its high sensitivity, the amount of RNA needed for asuccessful miRNA detection was in the range of 3−10 ng. In addition, a direct analysis of let-7 miRNA in serum was attempted. Unfortunately, persistently lower values with much larger variations (30%−50%) were obtained. The results were even worse when RNA extraction efficiency is taken into consideration. Evidently, the high protein content and other blood constituents in serum severely interfere with the detection of miRNA, probably by fouling the biosensor through nonspecific adsorption. Efforts in developing an antifouling interface while maintaining most of the attractive features of the biosensor are underway. At the present time, this work only serves as a proof-of- concept through rather labor-intensive manual operation. Nonetheless, as with other electronic/electrochemical bio- sensors, both low- and high-density sensor arrays can, in principle, be constructed by adopting the standard micro- fabrication technology and the complementary metal-oxide semiconductor technology that require no additional processes other than a simple switching mechanism. Two prominent examples demonstrating the power and capacity of the electronic/electrochemical detection systems are the electro- chemical microarray developed by combimatrix 34 and the Ion Proton Sequencer recently developed by Life Technologies. 35 For example, up to 12 million individal sensors can be fabricated on a single chip in the Ion Proton Sequencer. Compared to other label-free electrochemical biosensors, 12−15 the much simplified procedure implies that the throughput can also be significantly upscaled by upgrading the manual operation to a microfluidic cartridge. Of course, similar to all other hybridization-based systems, great care must be taken when profiling multiple miRNAs sinutaneously, since there is very little room to fine-tune the hybridization conditions for all miRNAs. |
回复此楼» 猜你喜欢
08工科求调剂290分
已经有12人回复
-
286求调剂
已经有16人回复
-
材料类284调剂
已经有24人回复
-
考研调剂-材料类-284
已经有14人回复
-
327求调剂
已经有11人回复
-
一志愿985初试354分生物调剂
已经有3人回复
-
一志愿2110，化学学硕310分，本科重点双非求调剂
已经有13人回复
-
353求调剂
已经有8人回复
-
308求调剂
已经有16人回复
-
334求调剂
已经有14人回复
yuankanxue
金虫 (著名写手)
分子玩家
- 应助: 38 (小学生)
- 金币: 1006.8
- 散金: 98
- 红花: 2
- 帖子: 1692
- 在线: 427.1小时
- 虫号: 595695
- 注册: 2008-09-08
- 性别: GG
- 专业: 波谱分析与成像分析
2楼2013-05-11 10:18:00
金狮水友
至尊木虫 (著名写手)
- 应助: 9 (幼儿园)
- 金币: 12278.2
- 散金: 50
- 红花: 13
- 沙发: 5
- 帖子: 1686
- 在线: 203小时
- 虫号: 1817809
- 注册: 2012-05-15
- 性别: GG
- 专业: 功能与智能高分子
3楼2013-05-11 12:28:03
金狮水友
至尊木虫 (著名写手)
- 应助: 9 (幼儿园)
- 金币: 12278.2
- 散金: 50
- 红花: 13
- 沙发: 5
- 帖子: 1686
- 在线: 203小时
- 虫号: 1817809
- 注册: 2012-05-15
- 性别: GG
- 专业: 功能与智能高分子
4楼2013-05-11 13:11:49
yuankanxue
金虫 (著名写手)
分子玩家
- 应助: 38 (小学生)
- 金币: 1006.8
- 散金: 98
- 红花: 2
- 帖子: 1692
- 在线: 427.1小时
- 虫号: 595695
- 注册: 2008-09-08
- 性别: GG
- 专业: 波谱分析与成像分析
5楼2013-05-11 14:54:26
cory0931
至尊木虫 (文坛精英)
巫山云
- 翻译EPI: 14
- 应助: 28 (小学生)
- 贵宾: 0.101
- 金币: 30151.9
- 散金: 13861
- 红花: 198
- 帖子: 10479
- 在线: 1003.7小时
- 虫号: 1652657
- 注册: 2012-02-29
- 性别: GG
- 专业: 临床兽医学
6楼2013-05-11 21:20:10
