²é¿´: 15298  |  »Ø¸´: 20
±¾Ìû²úÉú 1 ¸ö MolEPI £¬µã»÷ÕâÀï½øÐв鿴

stevenshi021

гæ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
sunzhengwang(gyesang´ú·¢): ½ð±Ò+1, ¹ÄÀø»ØÌûÓ¦Öú£¡ 2013-05-10 12:24:31
ÎÒÏëÊÇÄãµÄÏ´ÍÑÓÃÁËTE£â£õ£æ£æ£å£òÁË£¬ÕâÑùµ¼Ö£һáÉÔ΢±ä¸ß£¬£².£²Ö®Àà¶¼ÊÇ¿ÉÒÔ½ÓÊܵÄ
11Â¥2013-05-10 10:54:47
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

laojiu9878

ÖÁ×ðľ³æ (ÖªÃû×÷¼Ò)

¾ÅÅ£

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
ÓÐʱ¼ä·Ö¹â¹â¶È¼ÆÆ­ÈËÎÞµ×Ïß
µ«Ô¸È˳¤¾Ã
12Â¥2013-05-10 10:57:48
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

jiaoxiayoulu

гæ (СÓÐÃûÆø)

ÒýÓûØÌû:
4Â¥: Originally posted by bullfrog325 at 2013-05-09 22:03:31
ÎÒÕâÀïÁ¿³öÀ´Ò»Ö±ÊÇ2.1µÄÑù×Ó, ûÎÊÌâµÄ.

Õâ˵Ã÷´¿¶È»¹ÊǺܸߵģ¬ÊÇÂð£¿
13Â¥2013-05-10 11:01:59
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

louisa¿¡

Òø³æ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

Õâ¸öµÃ¿´¸÷¸ö²¨³¤´ú±íʲô£¬ÎÒÃÇÕâÒ»°ãA260/A280ÔÚ1.7¼¸£¬Êý¾Ý»¹ÊÇ¿ÉÒÔÓõġ£µ«Êdz¬¹ý2£¬ÎҾͲ»ÖªµÀÁË¡£¡£ÄãÊÇÓÃÊÔ¼ÁºÐÌáµÄÂ𣿣¿ÄãÔÙ½áºÏÊÔ¼ÁºÐ˵Ã÷Êé¿´¿´¡£¡£¡£¡£
14Â¥2013-05-10 11:31:41
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

chinatiger

ľ³æ (ÖøÃûдÊÖ)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï ¡ï ¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
sunzhengwang(gyesang´ú·¢): ½ð±Ò+2, ¹ÄÀø»ØÌûÓ¦Öú£¡ 2013-05-10 13:00:10
gyesang: »ØÌûÖö¥ 2013-05-10 13:00:24
sunzhengwang: ½ð±Ò+2, ¡ï¡ï¡ïºÜÓаïÖú 2013-05-10 15:16:13
1949stone: MolEPI+1, good 2013-05-11 20:30:21
Èç¹ûÔÚ2.1Ö®ÉÏ£¬ËµÃ÷¿ÉÄÜ´æÔÚDNAÎÛȾ¡£´ËÍ⣬µ¥´¿¿´±ÈÖµÒâÒå²»´ó£¬ÏÂÁÐÎÄ×Ö½ö¹©²Î¿¼£º
1. A260nm ÊǺËËá×î¸ßÎüÊÕ·åµÄÎüÊÕ²¨³¤£¬×î¼Ñ²âÁ¿ÖµµÄ·¶Î§Îª 0.1 ÖÁ 1.0¡£Èç¹û²»ÔÚ´Ë·¶Î§£¬Ï¡ÊÍ»òŨËõÑùÆ·£¬Ê¹ Ö®ÔÚ´Ë·¶Î§ÄÚ£»Èç¹ûÎü¹â¶ÈСÓÚ 0.05£¬¼ì²éÊÇ·ñ´æÔÚ²Ù×÷ÒòËØ£¨ÈçÒÆÒº²»×¼È·£¬ÑùÆ·ÄÚÓÐÐü¸¡ÎïµÈ£©Ó°Ïì¡£ DNA ÑùÆ·µÄ A260 Îü¹â¶ÈÖµÊÇ·ñ>0.1¡££¨Çë×¢Ò⣬Õâ¸öÖµ¸úÒÇÆ÷Î޹أ¬ºËËáµÄÎü¹â¶È±ØÐè´óÓÚ 0.1£¬ÆäÖµ²Å ÓÐЧºÍ¿É¿¿£¬ÒòΪÑùÆ·ÖеÄÔÓÖʺͿÅÁ£ÕâЩ²»´¿ÎïµÄ¸ÉÈÅͨ³£»á¶Ô¹âÓÐÒ»¶¨ÎüÊÕ£¬ÆäÖµ<0.1£©£»

2. A280nm, A270nm Êǵ°°××î¸ßÎüÊÕ·åµÄÎüÊÕ²¨³¤£¬±ÈÖµ¿É½øÐкËËáÑùÆ·´¿¶ÈÆÀ¹À£º´¿ DNA µÄ A260/A280 ±ÈֵΪ 1.8£¬´¿ RNA Ϊ 2.0¡£Èç¹û±ÈÖµµÍ£¬±íʾÊܵ½µ°°×£¨·¼Ïã×壩»ò·ÓÀàÎïÖʵÄÎÛȾ£¬ÐèÒª´¿»¯ÑùÆ·¡£±ÈÖµ=1.5 Ï൱ÓÚ 50£¥ µ°°×ÖÊ/DNA ÈÜÒº.·ÓµÄ×î´óÎüÊÕ·åÔÚ 270nm¡£

3. A230nm ÊÇ̼ˮ»¯ºÏÎï×î¸ßÎüÊÕ·åµÄÎüÊÕ²¨³¤£¬±ÈÖµ¿É½øÐкËËáÑùÆ·´¿¶ÈÆÀ¹À£º´¿ DNA ºÍ RNA µÄ A260/A230 ±ÈֵΪ 2.5¡£Èô±ÈֵСÓÚ 2.0 ±êÃ÷ÑùÆ·±»Ì¼Ë®»¯ºÏÎÌÇÀࣩ¡¢ÑÎÀà»òÓлúÈܼÁÎÛȾ£¬ÐèÒª´¿»¯ÑùÆ·¡£A230 ²úÉú ¸ºÖµÖ÷ÒªÊÇÓÉÓÚÔÚºÜµÍ DNA Ũ¶ÈµÄÈÜÒºÖеÄһЩÆäËû³É·ÖµÄ¸ÉÈÅËùµ¼Öµġ£ÔÚÏÂÒ»¸ö²â¶¨ÖУ¬ÐèÒª½µµÍÑù Æ·µÄÏ¡ÊͶȣ¬A230 µÄ¸ºÖµ»á±»Ð£Õý¡£

4. A320nm »ò A340nm Ϊ¼ì²âÈÜÒºÑùÆ·µÄ×Ç¶ÈºÍÆäËû¸ÉÈÅÒò×Ó¡£¸ÃÖµÓ¦¸Ã½Ó½ü0.0¡£Èç¹û²»ÊÇ£¬±êÃ÷ÈÜÒºÖÐÓÐÐü¸¡ÎÐèÒª´¿»¯ÑùÆ·¡£´¿ÑùÆ·µÄA320Ò»°ãÊÇ 0¡£

5. A260/A280ºÍA260/A230 ÊǺËËá´¿¶ÈµÄָʾֵ¡£´¿¶ÈºÃµÄDNA»òRNA£¬ÔÚpH7-8.5 ÏÂA260 / A280µÄ±ÈÖµÓ¦¸ÃÔÚ2.0 »ò2.5¡£´¿¾»µÄÑùÆ·±ÈÖµ´óÓÚ1.8£¨DNA£©»òÕß2.0£¨RNA£©¡£Èç¹û±ÈÖµµÍÓÚ1.8 »òÕß2.0£¬±íʾ´æÔÚµ°°×ÖÊ»òÕß·ÓÀàÎïÖʵÄÓ°Ïì¡£A230±íʾÑùÆ·ÖдæÔÚһЩÎÛȾÎÈç̼ˮ»¯ºÏÎï¡¢ÑΣ¨ëÒÑΣ©µÈ£¬½Ï´¿¾»µÄºËËáA260/A230µÄ±ÈÖµ´óÓÚ2.0 ¡£

µ± 0.5£¥BSAµ°°×ÖÊÎÛȾʱ£¬µ°°×ÎÛȾ»áµ¼ÖÂ260ºÍ280µÄÊýÖµ¶¼Ï½µ£¬Æä¾»½á¹ûÊÇ260/280±ÈֵϽµ£¬µ«260/280µÄ±ÈÖµ±ä»¯²¢²»ÏÔÖø£¬ÕýÈç·Ö×Ó¿Ë¡3Ëù˵¡£µ«µ°°×²ÐÁô»áµ¼ÖÂ230µÄÊýÖµÏÔÖøÉÏÉý£¬ÏÔÖøÓ°Ïì260/230µÄ±ÈÖµ¡£Ò²¾ÍÊÇ˵£¬Èç¹ûRNAÑùÆ·µÄ260/280£½1.7£¬260 /230£½0.5£¬ÄÇô¾ÍÓ¦¸Ã¿¼ÂÇÎÛȾԭÒò²»ÊÇëÒÑβÐÁô£¬¶øÊǵ°°×²ÐÁô.

ÆäËû£º
Ó÷ֹâ¹â¶È¼Æ²âÁ¿RNAʱ£¬ÓÃË®¶ø²»ÊÇÓÃTE »º³åҺϡÊÍRNAÑùÆ·»áÔì³ÉA260/A280±ÈֵϽµ¡£Ô­ÒòÊǵÍÀë×ÓÇ¿¶ÈºÍµÍpHÈÜÒº»áÔö¼Ó280 nm´¦µÄ¹âÎüÊÕÖµ¡£
¼ÓÂÈ·ÂÀëÐĺóÈ¡ÉÏÇåµÄʱºòǧÍò²»ÒªÌ°¶à £¬ÄÇÑù¾ÍºÜÈÝÒ×±»µ°°×ÎÛȾ£¬ËùÒÔÏÖÔÚÒ»°ãÈ¡400ul×óÓÒ¡£
µ±260/230<1ʱ£¬Ö»ÓÐÁ½ÖÖÇé¿ö¡£Ò»ÊÇëÒÑÎÎÛȾ£¬¶þÊǵ°°×ÎÛȾ¡£
15Â¥2013-05-10 11:39:21
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

springnamao

½ð³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
10Â¥: Originally posted by jiaoxiayoulu at 2013-05-10 10:50:29
È·¶¨Ã»´í£¿£¿£¿...

Ó¦¸Ãû´í£¬ÒªÃ´¾ÍÊǼǷ´ÁË
ÉúÈçÏÄ»¨
16Â¥2013-05-10 15:00:34
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

sunzhengwang

Ìú³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
11Â¥: Originally posted by stevenshi021 at 2013-05-10 10:54:47
ÎÒÏëÊÇÄãµÄÏ´ÍÑÓÃÁËTE£â£õ£æ£æ£å£òÁË£¬ÕâÑùµ¼Ö£һáÉÔ΢±ä¸ß£¬£².£²Ö®Àà¶¼ÊÇ¿ÉÒÔ½ÓÊܵÄ

Ò»°ãÔÚ2.04×óÓÒÅÇ»²
Ò»ÊÖÉú´æ£¬Ò»ÊÖÃÎÏë
17Â¥2013-05-10 15:18:56
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

sunzhengwang

Ìú³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
14Â¥: Originally posted by louisa¿¡ at 2013-05-10 11:31:41
Õâ¸öµÃ¿´¸÷¸ö²¨³¤´ú±íʲô£¬ÎÒÃÇÕâÒ»°ãA260/A280ÔÚ1.7¼¸£¬Êý¾Ý»¹ÊÇ¿ÉÒÔÓõġ£µ«Êdz¬¹ý2£¬ÎҾͲ»ÖªµÀÁË¡£¡£ÄãÊÇÓÃÊÔ¼ÁºÐÌáµÄÂ𣿣¿ÄãÔÙ½áºÏÊÔ¼ÁºÐ˵Ã÷Êé¿´¿´¡£¡£¡£¡£

trizolÌáµÄ
Ò»ÊÖÉú´æ£¬Ò»ÊÖÃÎÏë
18Â¥2013-05-10 15:19:30
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

É˺δ¦

ľ³æ (ÕýʽдÊÖ)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
260/280>2.0 Ò²ÓпÉÄÜÊÇRNA·¢Éú½µ½âÁË£¬ÄÚ²¿»ùÍÅÍâ¶£¬µ¼Ö¶Ô260nm²¨³¤µÄ¹âÎüÊÕÖµÔö´ó¡£Â¥Ö÷µçÓ¾½á¹ûÔõôÑù£¿
¶ÔÒ²°Õ£¬´íÒ²°Õ£»ÕýÒ²°Õ£¬ÄæÒ²°Õ£»Í¨Í¾Ò²ºÃ£¬Æéá«ÎÞ·Á¡­¡­µ«ÎÊ£¬ÎáÐļá·ñ£¿Èô¼á£¬×ÝʹÆç·£¬ÒÔÌúÐÄ£¬¹á´©¶ø¹ý£¬ÒàΪ´óµÀ£¡²»¼á£¬±ãÊÇ̹;£¬Ò²ÄѵÇÁÙ¾ø¶¥£¬¹ÛÏÕ·å
19Â¥2013-05-11 20:24:00
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

sunzhengwang

Ìú³æ (СÓÐÃûÆø)

ÒýÓûØÌû:
19Â¥: Originally posted by É˺δ¦ at 2013-05-11 20:24:00
260/280>2.0 Ò²ÓпÉÄÜÊÇRNA·¢Éú½µ½âÁË£¬ÄÚ²¿»ùÍÅÍâ¶£¬µ¼Ö¶Ô260nm²¨³¤µÄ¹âÎüÊÕÖµÔö´ó¡£Â¥Ö÷µçÓ¾½á¹ûÔõôÑù£¿

µçÓ¾½á¹û»¹²»´í£¬
Ò»ÊÖÉú´æ£¬Ò»ÊÖÃÎÏë
20Â¥2013-06-17 13:43:24
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ sunzhengwang µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] ¿¼Ñл¯Ñ§308·ÖÇóµ÷¼Á +10 ÄãºÃÃ÷ÌìÄãºÃ 2026-03-23 11/550 2026-03-25 10:23 by userper
[¿¼ÑÐ] BÇø¿¼Ñе÷¼Á +4 yqdszhdap£­ 2026-03-22 5/250 2026-03-25 08:51 by baoball
[¿¼ÑÐ] 292Çóµ÷¼Á +3 ¶ì¶ì¶ì¶î¶î¶î¶î¶ 2026-03-25 3/150 2026-03-25 07:45 by ms629
[¿¼ÑÐ] ÉúÎïѧѧ˶Çóµ÷¼Á +7 СÑò˯×ÅÁË? 2026-03-23 10/500 2026-03-25 02:24 by Çå·ç·÷Ñï¡£ m
[¿¼ÑÐ] ʳƷר˶ һ־Ը˫һÁ÷ 328 +3 xiaom99 2026-03-21 4/200 2026-03-24 21:20 by lailaisimei
[¿¼ÑÐ] Çóµ÷¼Á323²ÄÁÏÓ뻯¹¤ +3 1124361 2026-03-24 3/150 2026-03-24 20:24 by peike
[¿¼ÑÐ] 321Çóµ÷¼Á +4 Ymlll 2026-03-24 4/200 2026-03-24 14:44 by sprinining
[¿¼ÑÐ] 344Çóµ÷¼Á +3 desto 2026-03-24 3/150 2026-03-24 10:09 by ²«»÷518
[¿¼ÑÐ] Ò»Ö¾Ô¸±±¾©»¯¹¤´óѧ 070300 ѧ˶ 336·Ö Çóµ÷¼Á +7 vvÃÔ 2026-03-22 7/350 2026-03-23 23:44 by Txy@872106
[¿¼ÑÐ] 316Çóµ÷¼Á +7 ÁºÜçö© 2026-03-19 7/350 2026-03-23 16:21 by lingjue
[¿¼ÑÐ] 263Çóµ÷¼Á +6 yqdszhdap£­ 2026-03-22 9/450 2026-03-23 12:57 by yqdszhdap£­
[¿¼ÑÐ] 306Çóµ÷¼Á +5 À´ºÃÔËÀ´À´À´ 2026-03-22 5/250 2026-03-22 16:17 by BruceLiu320
[¿¼ÑÐ] Ò»Ö¾Ô¸»ªÖпƼ¼´óѧ071000£¬Çóµ÷¼Á +4 ÑØ°¶Óб´¿Ç6 2026-03-21 4/200 2026-03-22 07:21 by ilovexiaobin
[¿¼ÑÐ] 332Çóµ÷¼Á +3 ·ï»ËÔº¶¡Õæ 2026-03-20 3/150 2026-03-21 10:27 by luoyongfeng
[¿¼ÑÐ] 085601µ÷¼Á 358·Ö +3 zzzzggh 2026-03-20 4/200 2026-03-21 10:21 by luoyongfeng
[¿¼ÑÐ] Ò»Ö¾Ô¸ÎäÀí²ÄÁÏ305·ÖÇóµ÷¼Á +6 ÏëÉϰ¶µÄÀðÓã 2026-03-18 7/350 2026-03-21 01:03 by JourneyLucky
[¿¼ÑÐ] 304Çóµ÷¼Á +6 ÂüÊâ2266 2026-03-18 6/300 2026-03-21 00:32 by JourneyLucky
[¿¼ÑÐ] ÄϾ©´óѧ»¯Ñ§376Çóµ÷¼Á +3 hisfailed 2026-03-19 6/300 2026-03-20 23:43 by hisfailed
[¿¼ÑÐ] 0817 »¯Ñ§¹¤³Ì 299·ÖÇóµ÷¼Á ÓпÆÑо­Àú ÓжþÇøÎÄÕ +22 rare12345 2026-03-18 22/1100 2026-03-20 20:39 by zhukairuo
[¿¼ÑÐ] 086500 325 Çóµ÷¼Á +3 Áì´øÐ¡ÐÜ 2026-03-19 3/150 2026-03-20 18:38 by ¾¡Ë´Ò¢1
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û