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zengzhiyong

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title£ºEstablishment and application of a multiplex PCR method for detection of six swine virus DNA (PRV,PPV,PCV2) and RNA (PRRSV,CSFV,JEV) simultaneously

Abstract: According to the gene sequences in GenBank, six pairs of specific primers were designed to amplify fragments of the specific genes of PRV gE, PPV NS1, PCV2 ORF2, PRRSV ORF7, CSFV E2 and JEV E, respectively. The results of the multiplex PCR showed that the minimum nucleic acid detection amount of PRV, PPV, PCV2, PRRSV, CSFV and JEV were 6.6pg, 96pg, 12.9pg, 10.5pg, 51pg and 46pg, respectively, and both Swine Influenza Virus (SIV) and Escherichia coli (E.coli) were negative. The results of 103 clinical samples from Guizhou province revealed that the six viruses were positive in all pig farms in Guizhou province, and mixed infections were possible. The six-plex PCR method not only could simultaneously detect the infection of PRV, PPV, PCV, PRRSV, CSFV and JEV, but also could detect DNA and RNA virus synchronously, and could rapidly differentiate and diagnose the clinical samples of single infection or mixed infections of the six viruses.

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yuxinnick

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zengzhiyong: ½ð±Ò+5, ¡ïÓаïÖú 2013-04-27 15:49:08
Molecular and Cellular Probes
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2Â¥2013-04-27 10:57:23
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zengzhiyong

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2Â¥: Originally posted by yuxinnick at 2013-04-27 10:57:23
Molecular and Cellular Probes

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3Â¥2013-04-27 11:01:42
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zhoupeng87

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ÊÔÊÔVIRUS GENES Õâ¸ö
4Â¥2013-04-27 11:04:19
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w3195

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Ê×ÏÈ¿ÉÒÔÊÔÊÔjournal of veterinary diagnostic investigation
È»ºó¿ÉÊÇÊÔÊÔJournal of Virological Methods»òÕßJournal of Microbiological Methods
»¹¿ÉÒÔÊÔÊÔJOURNAL OF VETERINARY MEDICAL SCIENCE
5Â¥2013-04-27 20:03:25
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