| ²é¿´: 190 | »Ø¸´: 0 | |||
| µ±Ç°Ö÷ÌâÒѾ´æµµ¡£ | |||
jbllxy½ð³æ (СÓÐÃûÆø)
|
[½»Á÷]
Éú»¯ÊÔ¼Á µÄÇ󹺣¨ÄϲýÂÌºã¿Æ¼¼£©
|
||
|
ÊÔ¼Á 1£®PVP-40 ÒÒÏ©ßÁ¿©Íéͪ 2£®CTAB äå´úÊ®ÁùÍé»ùÈý¼×»ù°· 3£®EDTA-2Na ¶þË®ÒÒ¶þ°·ËÄÒÒËá¶þÄÆ 4£®Tris Base ÈýôǼ׻ù°±»ù¼×Íé¼î¡Á 5£®SDS Ê®¶þÍé»ù»ÇËáÄÆ 6£®E-ÛÏ»ùÒÒ´¼ 7£®ÈýÂȼ×Íé 8£®Òì±û´¼ 9£®ÒìÎì´¼ 10£®ÎÞË®ÒÒ´¼ 11£®ÇíÖ¬ÌÇ£¨Î÷°àÑÀ²ú£© 100¿Ë 1Æ¿ 12£®50¡ÁTAE»º³åÒº 500ml 2Æ¿ 13£®ä廯ÒÒ¶§£¨EB£© 14.Taq ø 5U/ul 15.dNTP 2.5mmol/L 16.±ê×¼DNA·Ö×ÓÁ¿±ê¼ÇÎDNA Marker£© 17.µçÓ¾ÉÏÑù»º³åÒº (Loading Buffcr) 18£®RNAø 19.PCR²úÎï»ØÊÕÊÔ¼ÁºÐ 20£®ÏÞÖÆÐÔÄÚÇÐøXmn1¼°·´Ó¦»º³åÒº 19£®ÒýÎï ´ó¶¹ 1£® lectin»ùÒò Lec-F1: 5¨@GCCCTCTACTCCACCCCCATCC3¨@ Lec-R1: 5¨@GCCCATCTGCAAGCCTTTTTGTG3¨@ 118bp 2. 35S-CTP4»ùÒò SC-F1: 5¨@TGATGTGATATCTCCACTGACG3¨@ SC-R1: 5¨@TGTATCCCTTGAGCCATGTTGT3¨@ 172bp 3. Cp4-epsps»ùÒò CE-F1: 5¨@CCTTCATGTTCGGCGGTCTCG3¨@ CE-R1: 5¨@GCGTCATGATCGGCTCGATG3¨@ 498bp 4. nosÖÕÖ¹×Ó»ùÒò Pnos-F1: 5¨@GAATCCTGTTGCCGGTCTTG3¨@ Pnos-R1: 5¨@TTATCCTAGTTTGCGCGCTA3¨@ 180bp 5. CaMV35SÆô¶¯×Ó»ùÒò 35S-F£º 5¨@GCTCCTACAAATGCCATCATTGC3¨@ 35S-R£º 5¨@GATAGTGGGATTGTGCGTCATCCC3¨@ 190bp ÃÞ»¨ 1£® Sad1»ùÒò s-F: CCAAAGGAGGTGCCTGTTCA s-R: TTGAGGTGAGTCAGAATGTTGTTC 108bp 2. nos ÖÕÖ¹×Ó»ùÒò Pnos-F1: 5¨@GAATCCTGTTGCCGGTCTTG3¨@ Pnos-R1: 5¨@TTATCCTAGTTTGCGCGCTA3¨@ 180bp 3. CaMV35SÆô¶¯×Ó»ùÒò 35S-F£º 5¨@GCTCCTACAAATGCCATCATTGC3¨@ 35S-R£º 5¨@GATAGTGGGATTGTGCGTCATCCC3¨@ 190bp 4. cry1AC»ùÒò¡¢cry1Ab»ùÒò¡¢ÒÔ¼°cry1ACÓëcry1AbÈںϻùÒòÐòÁÐͨÓÃÒýÎï cry1A-F£º5¨@GAAGGTTTGAGCAATCTCTAC3¨@ cry1A-R£º5¨@CGATCAGCCTAGTAAGGTCGT3¨@ 195bp ÑÌ²Ý 1£®35SÆô¶¯×Ó»ùÒò 35S-F£º 5¨@GCTCCTACAAATGCCATCATTGC3¨@ 35S-R£º 5¨@GATAGTCGGATTGTGCGTCATCCC3¨@ 195bp 2. nos ÖÕÖ¹×Ó»ùÒò nos-1: 5¨@GAATCCTGTTGCCGGTCTTG3¨@ nos-1: 5¨@TTATCCTAGTTTGCGCGCTA3¨@ 213bp 3. NPT11»ùÒò NPT-1£º 5¨@GCCCTGAATGAACTGCACGACCAGGC-3¨@ NPT-2£º 5¨@GCAGGCATCGCCATGGGTCACGACGA-3¨@ 411bp 5. ÑÌ²ÝÆÕͨ»¨Ò¶²¡¶¾Íâ¿Çµ°°×»ùÒòTMV-CP TMV1: 5¨@GTGTTCTTGTCATCAGCGTGGGC-3¨@ TMV2: 5¨@CACCGTTGCGTCGTCTACTCTACG-3¨@ 202bp 6. »Æ¹Ï»¨Ò¯²¡¶¾Íâ¿Çµ°°×»ùÒòCMV-CP CMV1: 5¨@ACCCAACCTTTGTAGGGAGTGAGCG-3¨@ CMV2: 5¨@ACATAGCAGAGATGGCGGCAACG-3¨@ 263BP 7. ÂíÁåÊíY²¡¶¾Íâ¿Çµ°°×»ùÒòPVY-CP PVY-1: 5¨@GATATTTCAAATACTCGGGCA-3¨@ PVY-2: 5¨@GCATAACGCGCTAAACCCAC-3¨@ 362bp 8. ÑÌ²ÝÆÕͨ»¨Ò¶²¡¶¾¸´ÖÆÃ¸»ùÒòTMV54KD T54D1: 5¨@GAGTTGTCTGGCATCATTGA-3¨@ T54D2: 5¨@ACAATGGTCAAAGCCGGGTA-3¨@ 295bp 9. ËÕÔÆ½ð¸Ë¾úɱ³æµ°°×»ùÒòBT BT-1: 5¨@CCCACTAGTTAACAATTTGATTGGA-3¨@ BT-2: 5¨@CCGGAAGCTTTAGGACTGTAGGT-3¨@ 299bp Îҵĵ绰ÊÇ13979125365 QQ 316770240 ÈçÓÐÏà¹ØÐÅÏ¢»òÕß»õÔ´£¬ÇëÓëÎÒ±¾ÈËÁªÏµ£¬Ð»Ð» [ Last edited by zhangwj on 2008-12-18 at 23:52 ] |
» ²ÂÄãϲ»¶
ѧ¿ÆÉúÎï354Çóµ÷¼Á
ÒѾÓÐ2È˻ظ´
´óÁ¬¹¤Òµ´óѧ ÉúÎïÖÊÄÜÔ´Óë²ÄÁÏרҵÕÐÊÕµ÷¼ÁÉú£¬ÓÐÐËȤ¼ÓÁªÏµ£º15732893021
ÒѾÓÐ0È˻ظ´
ÉúÎïÁ¦Ñ§Óë×éÖ¯¹¤³ÌѧÂÛÎÄÈóÉ«/·ÒëÔõôÊÕ·Ñ?
ÒѾÓÐ110È˻ظ´
ÉúÎï¹â×ÓѧÑо¿Ôº/¼¤¹âÉúÃü¿ÆÑ§½ÌÓý²¿ÖصãʵÑéÊÒ Ñо¿Éúµ÷¼Á
ÒѾÓÐ0È˻ظ´
´óÁ¬¹¤Òµ´óѧ ÉúÎïÖÊÄÜÔ´Óë²ÄÁÏרҵÕÐÊÕµ÷¼ÁÉú£¬ÓÐÐËȤ¼ÓÁªÏµ£º15732893021
ÒѾÓÐ0È˻ظ´
Çóµ÷¼Á£¡ÉúÎïÓëҽҩר˶
ÒѾÓÐ6È˻ظ´
´óÁ¬¹¤Òµ´óѧ ÉúÎïÖÊÄÜÔ´Óë²ÄÁÏרҵÕÐÊÕµ÷¼ÁÉú£¬ÓÐÐËȤ¼ÓÁªÏµ£º15732893021
ÒѾÓÐ0È˻ظ´
´óÁ¬¹¤Òµ´óѧ ÉúÎïÖÊÄÜÔ´Óë²ÄÁÏרҵÕÐÊÕµ÷¼ÁÉú£¬ÓÐÐËȤ½«¼òÀú·¢Ë͵½507432328@qq.com
ÒѾÓÐ0È˻ظ´
ÉúÎïѧ308·ÖÇóµ÷¼Á£¨Ò»Ö¾Ô¸»ª¶«Ê¦´ó£©
ÒѾÓÐ7È˻ظ´
´óÁ¬¹¤Òµ´óѧ ÉúÎïÖÊÄÜÔ´Óë²ÄÁÏרҵÕÐÊÕµ÷¼ÁÉú£¬ÓÐÐËȤ½«¼òÀú·¢Ë͵½507432328@qq.com
ÒѾÓÐ0È˻ظ´
ÉúÎïѧ308·ÖÇóµ÷¼Á£¨Ò»Ö¾Ô¸»ª¶«Ê¦´ó£©
ÒѾÓÐ10È˻ظ´














»Ø¸´´ËÂ¥
