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1£®PVP-40   ÒÒÏ©ßÁ¿©Íéͪ
2£®CTAB    äå´úÊ®ÁùÍé»ùÈý¼×»ù°·
3£®EDTA-2Na ¶þË®ÒÒ¶þ°·ËÄÒÒËá¶þÄÆ
4£®Tris Base  ÈýôǼ׻ù°±»ù¼×Íé¼î¡Á
5£®SDS     Ê®¶þÍé»ù»ÇËáÄÆ
6£®E-ÛÏ»ùÒÒ´¼
7£®ÈýÂȼ×Íé
8£®Òì±û´¼
9£®ÒìÎì´¼
10£®ÎÞË®ÒÒ´¼
11£®ÇíÖ¬ÌÇ£¨Î÷°àÑÀ²ú£©  100¿Ë   1Æ¿
12£®50¡ÁTAE»º³åÒº  500ml  2Æ¿
13£®ä廯ÒÒ¶§£¨EB£©
14.Taq ø  5U/ul
15.dNTP  2.5mmol/L
16.±ê×¼DNA·Ö×ÓÁ¿±ê¼ÇÎDNA Marker£©
17.µçÓ¾ÉÏÑù»º³åÒº (Loading Buffcr)
18£®RNAø
19.PCR²úÎï»ØÊÕÊÔ¼ÁºÐ
20£®ÏÞÖÆÐÔÄÚÇÐøXmn1¼°·´Ó¦»º³åÒº

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1£®        lectin»ùÒò
Lec-F1:  5¨@GCCCTCTACTCCACCCCCATCC3¨@
Lec-R1:  5¨@GCCCATCTGCAAGCCTTTTTGTG3¨@                       118bp
2.        35S-CTP4»ùÒò
SC-F1:   5¨@TGATGTGATATCTCCACTGACG3¨@
SC-R1:   5¨@TGTATCCCTTGAGCCATGTTGT3¨@                        172bp
3.        Cp4-epsps»ùÒò
CE-F1:   5¨@CCTTCATGTTCGGCGGTCTCG3¨@
CE-R1:   5¨@GCGTCATGATCGGCTCGATG3¨@                          498bp
4.        nosÖÕÖ¹×Ó»ùÒò
Pnos-F1:  5¨@GAATCCTGTTGCCGGTCTTG3¨@
Pnos-R1:  5¨@TTATCCTAGTTTGCGCGCTA3¨@                         180bp
5.        CaMV35SÆô¶¯×Ó»ùÒò
35S-F£º  5¨@GCTCCTACAAATGCCATCATTGC3¨@
35S-R£º  5¨@GATAGTGGGATTGTGCGTCATCCC3¨@                      190bp



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1£®        Sad1»ùÒò
s-F:     CCAAAGGAGGTGCCTGTTCA
s-R:     TTGAGGTGAGTCAGAATGTTGTTC                            108bp
2.  nos ÖÕÖ¹×Ó»ùÒò
Pnos-F1:   5¨@GAATCCTGTTGCCGGTCTTG3¨@
Pnos-R1:  5¨@TTATCCTAGTTTGCGCGCTA3¨@                         180bp
3.  CaMV35SÆô¶¯×Ó»ùÒò
35S-F£º  5¨@GCTCCTACAAATGCCATCATTGC3¨@
35S-R£º  5¨@GATAGTGGGATTGTGCGTCATCCC3¨@                      190bp
4.        cry1AC»ùÒò¡¢cry1Ab»ùÒò¡¢ÒÔ¼°cry1ACÓëcry1AbÈںϻùÒòÐòÁÐͨÓÃÒýÎï
cry1A-F£º5¨@GAAGGTTTGAGCAATCTCTAC3¨@
cry1A-R£º5¨@CGATCAGCCTAGTAAGGTCGT3¨@                          195bp


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1£®35SÆô¶¯×Ó»ùÒò
35S-F£º  5¨@GCTCCTACAAATGCCATCATTGC3¨@
35S-R£º  5¨@GATAGTCGGATTGTGCGTCATCCC3¨@                        195bp
2. nos ÖÕÖ¹×Ó»ùÒò
nos-1:   5¨@GAATCCTGTTGCCGGTCTTG3¨@
nos-1:   5¨@TTATCCTAGTTTGCGCGCTA3¨@                             213bp
3. NPT11»ùÒò
NPT-1£º  5¨@GCCCTGAATGAACTGCACGACCAGGC-3¨@
NPT-2£º  5¨@GCAGGCATCGCCATGGGTCACGACGA-3¨@                      411bp
5.        ÑÌ²ÝÆÕͨ»¨Ò¶²¡¶¾Íâ¿Çµ°°×»ùÒòTMV-CP
TMV1:    5¨@GTGTTCTTGTCATCAGCGTGGGC-3¨@
TMV2:    5¨@CACCGTTGCGTCGTCTACTCTACG-3¨@                        202bp
6.        »Æ¹Ï»¨Ò¯²¡¶¾Íâ¿Çµ°°×»ùÒòCMV-CP
CMV1:    5¨@ACCCAACCTTTGTAGGGAGTGAGCG-3¨@
CMV2:    5¨@ACATAGCAGAGATGGCGGCAACG-3¨@                         263BP
7.        ÂíÁåÊíY²¡¶¾Íâ¿Çµ°°×»ùÒòPVY-CP
PVY-1:   5¨@GATATTTCAAATACTCGGGCA-3¨@
PVY-2:   5¨@GCATAACGCGCTAAACCCAC-3¨@                             362bp
8.        ÑÌ²ÝÆÕͨ»¨Ò¶²¡¶¾¸´ÖÆÃ¸»ùÒòTMV54KD
T54D1:   5¨@GAGTTGTCTGGCATCATTGA-3¨@
T54D2:   5¨@ACAATGGTCAAAGCCGGGTA-3¨@                             295bp
9.        ËÕÔÆ½ð¸Ë¾úɱ³æµ°°×»ùÒòBT
BT-1:    5¨@CCCACTAGTTAACAATTTGATTGGA-3¨@
BT-2:    5¨@CCGGAAGCTTTAGGACTGTAGGT-3¨@                          299bp
  

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[ Last edited by zhangwj on 2008-12-18 at 23:52 ]
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