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liuqg1987

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专业: 生物化学

[求助] 哪个大师能帮小弟修改一段英语语句不胜感激

Since we found a significant change in growth of suspension cells (Fig. 3). The cell number was in a lower level before 4 h, but then rapidly raised with high budding rate and low mortality rate. When fermented for 16 h, the cell number achieved a maximum value of about ？ million/mL, which was closed to industrial level. However, increased ethanol exhibited a strong inhibition on yeast cells in the later period of fermentation, which led to a low budding rate and high mortality rate, thereby showing a decreased tendency in cell number. In addition, after 11 batches, a layer of enriched cells was observed in some place of carrier surface with the naked eye, which was not in consistent with Choi et al. who reported that it was difficult to accumulate cells through repeated batch processes. This result indicated that cell immobilization can promote cell growth and not seriously affected by sludge and suspended solid in the cassava meal medium.

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1楼 2013-04-17 18:39:22

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liuqg1987

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6楼: Originally posted by LT_FU at 2013-04-18 01:01:15
...，since we found a significant change in growth of suspension cells (Fig. 3). From the figure, we can see that the cell number is in a lower level at the first 4 hours of the fermentation. But, s ...

能帮我再看一段翻译么？
细胞数用血球计数板计数。将2.5 mL的样品和1mL的次亚甲基蓝加到100mL的容量瓶中，定容后静置2分钟。然后用计数板在显微镜下计数细胞。出芽率以出芽的细胞与总细胞比值表示，死亡率以染色的细胞与总细胞比值表示。
Cell number was estimated by blood counting chamber. 2.5 mL sample was added into100 mL volumetric flask with 1 mL methylene blue and was stood for 2 min after making 100 mL. Then cells including living and dead cells were counted by microscope with blood counting chamber. The budding rate was calculated as a ratio of budding cells to total cells. And death rate was calculated as a ratio of dead cells to total cells.