| 查看: 437 | 回复: 1 | ||
xiaotianh捐助贵宾 (小有名气)
|
[求助]
求助，翻译一段论文摘要。急！
|
| Etiologic diagnoses of lower respiratory tract infections (LRTI) have been relying primarily on bacterial cultures that often fail to return useful results in time. Although DNA-based assays are more sensitive than bacterial cultures in detecting pathogens, the molecular results are often inconsistent and challenged by doubts on false positives, such as those due to system- and environment-derived contaminations. Here we report a nationwide cohort study on 2986 suspected LRTI patients across P. R. China. We compared the performance of a DNA-based assay qLAMP (quantitative Loop-mediated isothermal AMPlification) with that of standard bacterial cultures in detecting a panel of eight common respiratory bacterial pathogens from sputum samples. Our qLAMP assay detects the panel of pathogens in 1047(69.28%) patients from 1533 qualified patients at the end. We found that the bacterial titer quantified based on qLAMP is a predictor of probability that the bacterium in the sample can be detected in culture assay. The relatedness of the two assays fits a logistic regression curve. We used a piecewise linear function to define breakpoints where latent pathogen abruptly change its competitive relationship with others in the panel. These breakpoints, where pathogens start to propagate abnormally, are used as cutoffs to eliminate the influence of contaminations from normal flora. With help of the cutoffs derived from statistical analysis, we are able to identify causative pathogens in 750 (48.92%) patients from qualified patients. In conclusion, qLAMP is a reliable method in quantifying bacterial titer. Despite the fact that there are always latent bacteria contaminated in sputum samples, we can identify causative pathogens based on cutoffs derived from statistical analysis of competitive relationship. |
回复此楼» 猜你喜欢
药学105500专硕289分求调剂
已经有3人回复
-
0703总分331求调剂
已经有15人回复
-
一志愿北京2，材料与化工308求调剂
已经有13人回复
-
318求调剂
已经有14人回复
-
285求调剂
已经有10人回复
-
312求调剂
已经有11人回复
-
273求调剂
已经有6人回复
-
生物学308分求调剂（一志愿华东师大）
已经有8人回复
-
生物学求调剂 一志愿沪9，326分
已经有3人回复
-
080500求调剂
已经有6人回复
2楼2013-04-13 01:05:30
10