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3Â¥: Originally posted by hookhans at 2013-03-28 14:10:09
Ask Rio~~~

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hookhans

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Ask Rio2016 (ID name) for help.
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jacksj: ½ð±Ò+30, ·­ÒëEPI+1, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸, Ê®·Ö¸Ðл£¡ 2013-03-28 19:57:27
ͨ¹ý3¡¯RACE¼¼Êõ³É¹¦¿Ë¡»ñµÃÁËÖíGCNFºÍSF-1µÄ3¡¯UTRÐòÁУ¬³¤¶È·Ö±ðΪ1816bpºÍ893bp.

The 3¡¯UTR sequence of GCNF and SF-1 genes were successfully cloned using 3 'RACE technology ,and the length of them is 1816 bp and 893 bp respectively.

Ó¦ÓÃMicroinspectorÈí¼þÔ¤²â²¢É¸Ñ¡ÁËÁ½¸ö¿ÉÄÜ×÷ÓÃÓÚGCNFºÍSF-1 3¡¯UTRµÄmiRNA£¬ËüÃÇ·Ö±ðÊÇssc-miR-181a¡¢ssc-miR-615¡£

Two miRNAs  which maybe act on the 3 'UTR of  GCNF and SF-1 were forecasted and screened using Microinspector software ,they are ssc-miR-181a and ssc-miR-615 respectively.

ÀûÓÃmiRNA QRT-PCR¼ì²âÁËÕâÁ½¸ömiRNAÔÚÖí¹Â´ÆÔçÆÚÅßÌ¥µÄ±í´ïÇé¿ö¡£

The expression level of the two miRNAs in early parthenogenetic embryos of pig is  detected using the miRNA QRT - PCR .

ͨ¹ýÓëGCNF¡¢SF-1µÄ±í´ï¹æÂɽøÐжԱȣ¬½á¹û·¢ÏÖmiRNAÓë°Ð»ùÒòÖ®¼ä±í´ï´æÔÚ×Å´ËÏû±Ë³¤µÄ¹ØÏµ£¬ÍƲâssc-miR-181aºÍssc-miR-615¿ÉÄÜ·Ö±ð²ÎÓëÁËGCNF¡¢SF-1ÔÚÔçÆÚÅßÌ¥·¢Óý¹ý³ÌÖеıí´ïµ÷¿Ø¡£

Compared to  the expression  pattern of  GCNF and SF-1,the results show that  there is a reciprocal relationship between the expression of  miRNA and its target genes,thus we predict that ssc-miR-181a and ssc-miR-615 maybe be involved in the expression regulation of   GCNF and SF-1 in the the process of early embryonic development.

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