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xiaoweiwei121

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zhangxingc: 金币+4, 鼓励热情回帖,小小的奖励一下 2013-03-22 09:01:26
查到你的那篇文献了
说实话,我感觉是他写错了,当然我以前也没做个这个pcr
个人认为应该是triton,具体的理由就是,我刚去翻看了另外一篇文献,里边有这么一段话
“Several approaches have been proposed to overcome this problem including additives usage in different PCR procedures (2, 6). Different additives were utilized to amplify GC-rich regions including betaine (N,N,N- trimethylglycin), dimethyl sulfoxide (DMSO), formamide, glycerin, glycerol, dithiothreitol (DTT), Bovine Serum Albumin (BSA), non-ionic detergents such as Triton X-100, nucleotide analogs 7-deaza dGTP, ethylene glycol and 1, 2-propanediol, and reducing agents such as β-mercapto-ethanol (1–9). These additives not only prevent secondary structure formation of both templates and primers, but also increase the annealing chance of primers to plate strands at temperatures lower than the melting temperature of primers (1, 3, 5, 7, 9).

文章地址
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558226/
11楼2013-03-22 06:55:49
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dongniaoxin

铁杆木虫 (正式写手)

响当当的木头

应该是tri ton 吧

[ 发自手机版 http://muchong.com/3g ]
没有梦就走,没有错就不向谁低头
12楼2013-03-22 07:37:45
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lvxingan1989

金虫 (小有名气)

引用回帖:
10楼: Originally posted by xiaoweiwei121 at 2013-03-22 06:31:42
promega是个公司哦...

好吧...
实验一般叫人很纠结..
13楼2013-03-22 10:13:34
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dearyangcong

金虫 (初入文坛)

是TritonX-100。一种表面活性剂。一般市面上都卖10%的,如果配0.1%的一般加10ml。可以去promega的网站上确认一下

[ 发自手机版 http://muchong.com/3g ]
14楼2013-03-22 10:38:26
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anm0316

金虫 (小有名气)

引用回帖:
14楼: Originally posted by dearyangcong at 2013-03-22 10:38:26
是TritonX-100。一种表面活性剂。一般市面上都卖10%的,如果配0.1%的一般加10ml。可以去promega的网站上确认一下

谢谢
15楼2013-03-23 10:59:19
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anm0316

金虫 (小有名气)

引用回帖:
11楼: Originally posted by xiaoweiwei121 at 2013-03-22 06:55:49
查到你的那篇文献了
说实话,我感觉是他写错了,当然我以前也没做个这个pcr
个人认为应该是triton,具体的理由就是,我刚去翻看了另外一篇文献,里边有这么一段话
“Several approaches have been proposed to o ...

谢谢
16楼2013-03-23 11:01:10
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