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anm0316

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[求助] Tryton是什么东西?

看一篇文献写PCR体系中写到: 2.5 mM MgCl 2 , 0.1% Tryton (Promega, Madison, Wis.),其中的Tryton是什么意思啊?
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xiaoweiwei121

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2楼: Originally posted by lvxingan1989 at 2013-03-21 10:44:10
tryton是个牌子貌似,而Promega才是有效物质

promega是个公司哦
10楼2013-03-22 06:31:42
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xiaoweiwei121

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zhangxingc: 金币+4, 鼓励热情回帖,小小的奖励一下 2013-03-22 09:01:26
查到你的那篇文献了
说实话,我感觉是他写错了,当然我以前也没做个这个pcr
个人认为应该是triton,具体的理由就是,我刚去翻看了另外一篇文献,里边有这么一段话
“Several approaches have been proposed to overcome this problem including additives usage in different PCR procedures (2, 6). Different additives were utilized to amplify GC-rich regions including betaine (N,N,N- trimethylglycin), dimethyl sulfoxide (DMSO), formamide, glycerin, glycerol, dithiothreitol (DTT), Bovine Serum Albumin (BSA), non-ionic detergents such as Triton X-100, nucleotide analogs 7-deaza dGTP, ethylene glycol and 1, 2-propanediol, and reducing agents such as β-mercapto-ethanol (1–9). These additives not only prevent secondary structure formation of both templates and primers, but also increase the annealing chance of primers to plate strands at temperatures lower than the melting temperature of primers (1, 3, 5, 7, 9).

文章地址
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558226/
11楼2013-03-22 06:55:49
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