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一篇2011年的脱细胞器官组织工程综述文献
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标题：Whole-Organ Tissue Engineering: Decellularization and Recellularization of Three-Dimensional Matrix Scaffolds 摘要：The definitive treatment for end-stage organ failure is orthotopic transplantation. However, the demand for transplantation far exceeds the number of available donor organs. A promising tissue-engineering/regenerative-medicine approach for functional organ replacement has emerged in recent years. Decellularization of donor organs such as heart, liver, and lung can provide an acellular, naturally occurring three-dimensional biologic scaffold material that can then be seeded with selected cell populations. Preliminary studies in animal models have provided encouraging results for the proof of concept. However, significant challenges for three-dimensional organ engi-neering approach remain. This manuscript describes the fundamental con-cepts of whole-organ engineering, including characterization of the extra-cellular matrix as a scaffold, methods for decellularization of vascular organs, potential cells to reseed such a scaffold, techniques for the recellularization process and important aspects regarding bioreactor design to support this approach. Critical challenges and future directions are also discussed. 主要内容：综述了细胞外基质作为生物支架的基础知识、整个器官的脱细胞方法、再细胞化的种子细胞来源和再细胞化方法。 参考文献：163篇[ Last edited by fool_proof on 2013-2-21 at 18:09 ] |
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2013-02-21 17:55:47, 429.77 K
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