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[求助]
Origami™ B(DE3) 菌株 是否有lacZ活性？
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如题； 我买的Origami™ B(DE3) Competent Cells，介绍里面说是一株BL21的LacZY基因缺失菌株。小弟想知道，该菌株的lacZ活性还存在不？ 附Origami™ B(DE3) Competent Cells的介绍和基因型： Origami™ B host strains carry the same mutations in trxB and gor as the original Origami strains, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels by adjusting the concentration of IPTG. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner™, and Origami strains in one strain background. The mutations in trxB and gor are selectable on kanamycin and tetracycline, respectively; therefore, these strains cannot be used with plasmids that can only selected with kanamycin or tetracycline. These strains also include the lon and ompT deficiences of BL21, which increase protein stability. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. Genotype: F S (r m ) -ompT hsdB B-B-galdcm lacY1 ahpC (DE3) gor522:: Tn10 trxB (Kan , Tet ) |
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2楼2013-01-17 16:21:20