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jingshan508至尊木虫 (职业作家)
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[求助]
磷酸葡萄糖转移酶系统(PTS)
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| 有没有关于磷酸葡萄糖转移酶系统(PTS)[color=Red]的中文综述，好像找不到中文的，刚入门什么都不懂。只想深入了解一下，看了百科太少了。 |
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susizheng
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我們是糖 甜到憂傷
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看天: 金币+3, BioEPI+1, 鼓励 常来 2012-12-06 16:42:59
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看天: 金币+3, BioEPI+1, 鼓励 常来 2012-12-06 16:42:59
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这个国内应该是研究比较少了，国外研究比较早，综述方面应该还没有，不过可以提供一些研究方面的内容， 比较早的是dupont公司的1,3-丙二醇项目有涉及到PTS部分的改造；以下文献都有涉及到，如果详细了解可以参考其引文 [1]Nakamura C E ,Whited GM. Metabolic engineering for the microbial production of 1, 3 propanediol[J]. Current Opinion in Biotechnology, 2003, 14: 454 -459. [2]Robert Westervelt. DuPont, Tate & Lyle Form joint venture for propanediol production[J]. Chemical Week, 2004, 166(18): 101 然后就是Myriant公司的生物丁二酸项目中也涉及到PTS部分的研究，相关文献可专利你也可以了解下，要想了解PTS详细内容，也只能是你去看以下论文的相关引文了，我只是抛砖引玉。 2009-Reengineering Escherichia coli for Succinate Production in Mineral Salts Medium PNAS-2009-Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli 国内研究PTS相关的很少，目前我了解的只有天津工生所的张学礼研究员，其有Myriant公司背景，他的生物丁二酸项目也有涉及到PTS系统改造的内容，2012年刚发了一篇文章，你可以自己去查查看。我就不帮忙了。 |
3楼2012-12-06 09:13:45
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wizardfan: 金币+2, 光贴文字帮助有限，能给出文献出处更好 2012-12-06 07:35:01
wizardfan: 金币+2, 光贴文字帮助有限，能给出文献出处更好 2012-12-06 07:35:01
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英文的不知道对你有没有帮助 PEP group translocation, also known as the phosphotransferase system or PTS, is a distinct method used by bacteria for sugar uptake where the source of energy is from phosphoenolpyruvate (PEP). It is known as multicomponent system that always involves enzymes of the plasma membrane and those in the cytoplasm. An example of this transport is found in E. coli cells. The system was discovered by Saul Roseman in 1964.[1] The phosphotransferase system is involved in transporting many sugars into bacteria, including glucose, mannose, fructose and cellobiose. PTS sugars can differ between bacterial groups, mirroring the most suitable carbon sources available in the environment every group evolved. The phosphoryl group on PEP is eventually transferred to the imported sugar via several proteins. The phosphoryl group is transferred to the Enzyme E I (EI), Histidine Protein (HPr, Heat-stable Protein) and Enzyme E II (EII) to a conserved histidine residue, whereas in the Enzyme E II B (EIIB) the phosphoryl group is usually transferred to a cysteine residue and rarely to a histidine.[2] In the process of glucose PTS transport specific of enteric bacteria, PEP transfers its phosphoryl to a histidine residue on EI. EI in turn transfers the phosphate to HPr. From HPr the phosphoryl is transferred to EIIA. EIIA is specific for glucose and it further transfers the phosphoryl group to a juxtamembrane EIIB. Finally, EIIB phosphorylates glucose as it crosses the plasma membrane through the transmembrane Enzyme II C (EIIC), forming glucose-6-phosphate.[2] The benefit of transforming glucose into glucose-6-phosphate is that it will not leak out of the cell, therefore providing a one-way concentration gradient of glucose. The HPr is common to the phosphotransferase systems of the other substrates mentioned earlier, as is the upstream EI.[3] Proteins downstream of HPr tend to vary between the different sugars. The transfer of a phosphate group to the substrate once it has been imported through the membrane transporter prevents the transporter from recognizing the substrate again, thus maintaining a concentration gradient that favours further import of the substrate through the transporter. With the glucose phosphotransferase system, the phosphorylation status of EIIA can have regulatory functions. For example, at low glucose concentrations phosphorylated EIIA accumulates and this activates membrane-bound adenylate cyclase. Intracellular cyclic AMP levels rise and this then activates CAP (catabolite activator protein), which is involved in the catabolite repression system, also known as glucose effect. When the glucose concentration is high, EIIA is mostly dephosphorylated and this allows it to inhibit adenylate cyclase, glycerol kinase, lactose permease, and maltose permease. Thus, as well as the PEP group translocation system being an efficient way to import substrates into the bacterium, it also links this transport to regulation of other relevant proteins. It is an active transport. After the translocation, the metabolites transported are modified. |
2楼2012-12-06 01:36:24
jingshan508
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4楼2012-12-06 12:01:13