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开心就好4453

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[交流] 端粒酶提取

端粒酶提取需要注意哪些特别的细节吗/
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harmony999

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开心就好4453(金币+1): 谢谢参与
有专用的细胞裂解液,低温下离心
2楼2012-11-27 17:02:52
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开心就好4453

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引用回帖:
2楼: Originally posted by harmony999 at 2012-11-27 17:02:52
有专用的细胞裂解液,低温下离心

您好,你说的专用的细胞裂解液是自己配的还是有卖成套包装的?
3楼2012-11-28 11:36:42
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harmony999

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★ ★ ★ ★
开心就好4453: 金币+4 2012-11-29 15:52:49
你可以参考一下这篇文献是混合溶液,要自己购买CHAP
Amplified Telomerase Analysis by Using Rotating Magnetic Particles: The Rapid and Sensitive Detection of Cancer Cells
Cells and tissues: HeLa and 293-kidney cancer cell lines, and NHF
cells, were stored as pellets at 808 C until extraction. Lung tissue
samples were obtained from patients with lung carcinoma. The di-
agnosis of each tissue sample was histologically determined by a
pathologist. All surgical specimens were kept at  808 C until ex-
traction. Cell pellets and tissue samples were lysed by using the
CHAPS Lysis buffer (Intergren Co.).
4楼2012-11-28 17:08:00
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开心就好4453

铜虫 (小有名气)


引用回帖:
4楼: Originally posted by harmony999 at 2012-11-28 17:08:00
你可以参考一下这篇文献是混合溶液,要自己购买CHAP
Amplified Telomerase Analysis by Using Rotating Magnetic Particles: The Rapid and Sensitive Detection of Cancer Cells
Cells and tissues: HeLa and 293 ...

您好,方便告诉下您的QQ吗?谢谢
5楼2013-03-18 11:32:20
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