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Microbial fuel cells (MFCs) have gained increasing interest as a new sustainable power source, because they are capable of degrading organic fuels including organic wastes to harvest electricity.How-ever, the sluggish electron transfer between bacteria cells and electrode is the main blockage to a desired power density for broad practical applications . Up to date, most efforts in improvement of MFC power density have been focused on nanoengineering and/or modify-ing electrode materials for high speci fic surface area and optimization of device con figuration and/or operation modes for better mass/ electron transport. It is very challenging to signi ficantly enhance the direct electron transfer process between bacteria cells and electrode for high power density . We have reported that Escherichia coli evolve to possess direct electron transfer with the supporting electrode after a long term discharge. The evolved cells form rougher surface with larger pores on the outer membrane than the original cells to enhance the transport of endogenous electronic shuttles for a fast medi-ated electron transfer. Similarly, MFCs inoculated with a mixed culture of Shewanella oneidensis MR-1 mutant library naturally select the cells with rough surface morphology, and these enriched mutants are impaired in cell surface polysaccharide biosynthesis. Some cationic reagents can perforate the cell's outer membrane to have weak antibac-terial effect and have been used in drug delivery systems. In this study, three reagents, chitosan, ethylenediaminetetraacetic acid (EDTA)and polyethyleneimine (PEI) were explored to perforate Pseudomonas aeruginosa (P. aeruginosa ) cells, and their electron transfer process in MFCs was further investigated. |
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2楼2012-11-07 10:05:36
yangzhehao
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