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给大家推荐一个挑晶体的好东西已有24人参与
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最近发现一个叫MiTeGen的公司买一些挑晶体的工具， 我隔壁实验室买了一盒tips，我去看了下，非常好用。 下面是这个公司的主页。 http://www.mitegen.com/ 下面这个链接是我隔壁实验室用的。我正准备买一套。 http://www.mitegen.com/products/micromeshes/micromeshes.shtml 有了这个我们就不用自己拉玻璃丝了，而且不用胶。tip头上是个像筛子一样的，有不同的大小。特别有利于挑小晶体，针状晶体和片状晶体。 首先要用油 （http://www.mitegen.com/mic_catalog.php?c=nvhoil）把晶体盖住，然后用这个tip伸到晶体下面，把晶体从油里面捞出来就搞定了。 唯一的缺点就是片状晶体在center的时候会比较难。  tips.jpg |
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看看网站上的介绍. http://www.mitegen.com/mic_catalog.php?c=nvhoil Products: NVH Oil This very high viscosity oil is suitable for room and variable-temperature diffraction measurements. Crystal motion during data collection is minimized, even when thick layers are used to prevent dehydration. This oil is very effective in removing external solvent to prevent ice ring formation during cooling. Move your crystal back and forth in the oil, transfer to fresh oil, and repeat until you no longer see solvent trails and the crystal becomes nearly invisible. Many protein crystals prepared in this way can be slowly cooled to T=100 K without appreciable degradation of diffraction properties, allowing data collection at arbitrary temperatures. Unlike Paratone-N oil, this oil does not form diffraction rings when cooled. 4 fl. oz. bottle. A note on using oils: After crystals have been transfered to oil they may seem to disappear, suggesting that they have dissolved. But don't worry: your crystal is still intact. The refractive index of oil (n=1.5) is very close to that of protein crystals, and much closer than that of water (n=1.3). Once the oil has displaced all the water from the crystal's surface, the interface between the oil and crystal surface will reflect very little light, making the crystal hard to see. Just adjust your illumination and look carefully. |
22楼2012-10-27 10:54:08
songxiao60
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3楼2012-10-23 09:12:56
huahua1216
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4楼2012-10-23 09:16:28
yytsnake
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5楼2012-10-23 09:36:49