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justdilo

木虫 (著名写手)

[求助] 帮我看看评审专家的这段话是什么意思?

Reviewer #3:
Wording: The PCR amplification products were confirmed seperated on a 1.0% agarose gel and extracted using the TIANgel Midi Purification Kit (Tiangen Biotech, China) based methods. The deseired bands were gel purified (kit?) and then cloned with pEASY-T1 vector.
原文:
The PCR amplification products were confirmed on a 1.0% agarose gel and extracted using the TIANgel Midi Purification Kit (Tiangen Biotech, China) based methods. The desired PCR bands were cloned with pEASY-T1 vector.
红色是差异部分。
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glassman782

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justdilo: 金币+5, 就你一个热心人,谢谢。 2012-11-02 11:11:48
问你是不是先凝胶纯化再用pEASY-T1 载体r克隆
2楼2012-10-12 22:55:10
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