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seq.cn

铜虫 (初入文坛)


[资源] 【资料】An efficient ChIP protocol

An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in Arabidopsis plants
Chromatin immunoprecipitation (ChIP) is a powerful tool for the characterization of covalent histone modifications and DNA–histone
interactions in vivo. The procedure includes DNA–histone cross-linking in chromatin, shearing DNA into smaller fragments,
immunoprecipitation with antibodies against the histone modifications of interest, followed by PCR identification of associated DNA sequences. In this protocol, we describe a simplified and optimized version of ChIP assay by reducing the number of experimental steps and isolation solutions and shortening preparation times. We include a nuclear isolation step before chromatin shearing, which provides a good yield of high-quality DNA resulting in at least 15 lg of DNA from each immunoprecipitated sample (from 0.2 to 0.4 g
of starting tissue material) sufficient to test Z25 genes of interest. This simpler and cost-efficient protocol has been applied for histone-modification studies of various Arabidopsis thaliana tissues and is easy to adapt for other systems as well.[ Last edited by silicare on 2012-11-1 at 10:44 ]
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  • 附件 1 : 植物ChIP.pdf
  • 2012-10-08 15:44:54, 599.17 K

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lwy2001

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★★★★★ 五星级,优秀推荐

好文章,真发愁无法下载,楼主好人。
8楼2014-08-07 14:13:58
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Ning_Wang

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学习一下,感谢分享!
5楼2012-11-01 10:28:52
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jiangronglu

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★★★★★ 五星级,优秀推荐

楼主这个ChIP.PDF.下载不了,给我传一个吧,谢谢!
7楼2013-07-13 10:12:19
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简单回复
silicare3楼
2012-10-08 17:00   回复  
五星好评  顶一下,感谢分享!
mlw09276楼
2013-01-05 11:36   回复  
五星好评  顶一下,感谢分享!
果壳4楼
2012-10-09 16:23   回复  
五星好评  顶一下,感谢分享!
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