| 查看: 677 | 回复: 7 | |||
| 【奖励】 本帖被评价7次，作者seq.cn增加金币 5.6 个 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
[资源]
【资料】An efficient ChIP protocol
|
|||
|
An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in Arabidopsis plants Chromatin immunoprecipitation (ChIP) is a powerful tool for the characterization of covalent histone modifications and DNA–histone interactions in vivo. The procedure includes DNA–histone cross-linking in chromatin, shearing DNA into smaller fragments, immunoprecipitation with antibodies against the histone modifications of interest, followed by PCR identification of associated DNA sequences. In this protocol, we describe a simplified and optimized version of ChIP assay by reducing the number of experimental steps and isolation solutions and shortening preparation times. We include a nuclear isolation step before chromatin shearing, which provides a good yield of high-quality DNA resulting in at least 15 lg of DNA from each immunoprecipitated sample (from 0.2 to 0.4 g of starting tissue material) sufficient to test Z25 genes of interest. This simpler and cost-efficient protocol has been applied for histone-modification studies of various Arabidopsis thaliana tissues and is easy to adapt for other systems as well.[ Last edited by silicare on 2012-11-1 at 10:44 ] |
回复此楼» 本帖附件资源列表
-
欢迎监督和反馈：小木虫仅提供交流平台，不对该内容负责。
本内容由用户自主发布，如果其内容涉及到知识产权问题，其责任在于用户本人，如对版权有异议，请联系邮箱：xiaomuchong@tal.com - 附件 1 : 植物ChIP.pdf
2012-10-08 15:44:54, 599.17 K
» 收录本帖的淘帖专辑推荐
 国外专业电子书 |
» 猜你喜欢
310求调剂
已经有14人回复
-
290调剂生物0860
已经有28人回复
-
山东双非院校考核超级无底线，领导幸灾乐祸，教师遭殃恐
已经有4人回复
-
279学硕食品专业求调剂院校
已经有14人回复
-
0831生医工第一轮调剂失败求助
已经有16人回复
-
电子信息270求调剂
已经有18人回复
-
食品与营养（0955）271求调剂
已经有5人回复
-
293求调剂
已经有11人回复
-
322求调剂
已经有13人回复
-
272分材料子求调剂
已经有50人回复
» 本主题相关价值贴推荐，对您同样有帮助:
- 【2014-04-28】ICESS2014 第11届嵌入式软件和系统国际会议
已经有145人回复
- ICESS2014 第11届嵌入式软件和系统国际会议征稿 （EI 检索）
已经有2人回复
- 代发：伦敦大学学院EE招收公派PhD两名
已经有2人回复
- 求助Nature Protocols
已经有1人回复
7楼2013-07-13 10:12:19
5楼2012-11-01 10:28:52
8楼2014-08-07 14:13:58
简单回复
silicare3楼
2012-10-08 17:00
回复
五星好评 顶一下，感谢分享！
mlw09276楼
2013-01-05 11:36
回复
五星好评 顶一下，感谢分享！
果壳4楼
2012-10-09 16:23
回复
五星好评 顶一下，感谢分享！
