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【答案】应助回帖
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爱与雨下: 金币+1 2012-10-05 10:40:45
huojinlong8610: 金币+200, 翻译EPI+1, ★★★★★最佳答案, 挺好的，加油，下次我们继续合作，谢谢！ 2012-10-06 21:42:26
1. 这段中文让人读的喘不过来气啊，断句不好，层次不是很清楚。因为我不是很专业，所以不清楚文字是否准确简练。
2.如果作为摘要，我觉得更需要将文字再琢磨琢磨，一般摘要需要十分精练，不能什么都说。如果是discussion中总结，也过于繁琐。
3.上边英文翻译省去了很多中文中说的文字。符合摘要形式，但是可能也丢掉很多重点吧。因为不知道重点在那里（这个只有你自己清楚），所以不知道要如何翻译。
4.强烈建议，把中文写好，放在上述中文下面，呵呵。否则，是在没办法翻译啊。
本不想翻译的，可是已经翻译了一部分，所以只能继续了，没有什么润色。下面是按照上述中文翻译的，结果部分一些主语不明，所以没有翻译。
To explore the capability of interleukin-6/interferon-α (IL6/IFNα) fusion protein as/being as an immunoadjuvant, the coding sequences of IL6 and IFN were cloned. Considering the characteristics of these coding sequences and the sequence of prokaryotic expression vector pET32a, the mature peptide sequences were amplified using the specific primer(s) designed with restriction enzyme sites, Linker sequence and His tag, and then transfected into E.coli DH5αafter linked with pMD18-T, respectively. The plasmids were extracted and restricted, and then linked via a hydrophilic and low-charge Linker sequence, which was then restricted simultaneously to construct the recombinant plasmid of pMD18-IL6-IFNα. This recombinant plasmid and expression vector pET32a were restricted and linked to construct the pET32-IL6-IFNα plasmid. The plasmid was transformed into E.coli DH5α and Rosetta (DE3), and then expressed under different concentrations' IPTG and times. The results showed that the pET32-IL6-IFNαwas constructed successfully. …. The results show that the expression plasmid is successfully constructed and IL6-IFNα protein is expressed in E.coli. This study will be a foundation for further study and application of IL6-IFNα as a novel efficient immunoadjuvant. |
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