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huojinlong8610金虫 (小有名气)
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[求助]
翻译过的请润色，没有翻译的请翻译为英文
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该段话有的翻译过了，请润色，没有翻译的请翻译 为获得猪白细胞介素6和α干扰素双重活性的融合蛋白，研究其作为高效免疫佐剂的可行性，本研究首先克隆出猪白细胞介素6（IL6）和α干扰素（IFNα）基因的编码区序列，综合考虑IL6和IFNα的编码区序列以及原核表达载体pET32a序列，设计带有限制性酶切位点、Linker序列以及His标签的特异性引物来扩增猪IL6和IFNα基因的成熟肽基因序列，分别与pMD18-T载体连接后转化大肠杆菌E.coli DH5α，提取质粒进行酶切后通过柔性亲水低电荷的Linker接头将二者串联后，通过同时酶切并连接构建重组质粒pMD18-IL6-IFNα，将该重组质粒和表达载体pET32a同时酶切并连接构建pET32-IL6-IFNα重组质粒，依次转化E.coli DH5α和Rosetta (DE3)感受态细胞，并经不同浓度的IPTG以及不同时间进行诱导表达。结果成功构建了pET32-IL6-IFNα重组表达载体，SDS-PAGE检测在Rosetta (DE3)中得到了高效表达，目的蛋白的相对分子量为44.96 KDa，与理论预期值一致，超声波后SDS-PAGE检测发现蛋白主要以不溶性的包涵体形式存在。本研究成功构建了猪白细胞介素6和α干扰素的融合表达载体，并为利用该蛋白作为高效免疫制剂的应用奠定了基础。 To explore the feasibility of using fusion protein of interleukin-6 (IL6) and interferon-α (IFNα) as an immunoadjuvant, the mature peptide genes of IL6 and IFNα were cloned and linked via a hydrophilic and low charge linker sequence，and subcloned to pET32α for prokaryotic expression. The recombinant plasmid was transformed into E.coli DH5α and Rosetta (DE3), then induced with different concentrations' IPTG. SDS-PAGE results showed that recombinant proteins were expressed in inclusion bodies with molecular weight of 44.96 KDa. The results show that the expression plasmid is successfully constructed and IL6-IFNα protein is expressed in E.coli. This study will be a foundation for further study and application of IL6-IFNα as a novel efficient immunoadjuvant. |
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2楼2012-10-04 11:03:56
【答案】应助回帖
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爱与雨下: 金币+1 2012-10-05 10:40:45
huojinlong8610: 金币+200, 翻译EPI+1, ★★★★★最佳答案, 挺好的，加油，下次我们继续合作，谢谢！ 2012-10-06 21:42:26
爱与雨下: 金币+1 2012-10-05 10:40:45
huojinlong8610: 金币+200, 翻译EPI+1, ★★★★★最佳答案, 挺好的，加油，下次我们继续合作，谢谢！ 2012-10-06 21:42:26
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1. 这段中文让人读的喘不过来气啊，断句不好，层次不是很清楚。因为我不是很专业，所以不清楚文字是否准确简练。 2.如果作为摘要，我觉得更需要将文字再琢磨琢磨，一般摘要需要十分精练，不能什么都说。如果是discussion中总结，也过于繁琐。 3.上边英文翻译省去了很多中文中说的文字。符合摘要形式，但是可能也丢掉很多重点吧。因为不知道重点在那里（这个只有你自己清楚），所以不知道要如何翻译。 4.强烈建议，把中文写好，放在上述中文下面，呵呵。否则，是在没办法翻译啊。 本不想翻译的，可是已经翻译了一部分，所以只能继续了，没有什么润色。下面是按照上述中文翻译的，结果部分一些主语不明，所以没有翻译。 To explore the capability of interleukin-6/interferon-α (IL6/IFNα) fusion protein as/being as an immunoadjuvant, the coding sequences of IL6 and IFN were cloned. Considering the characteristics of these coding sequences and the sequence of prokaryotic expression vector pET32a, the mature peptide sequences were amplified using the specific primer(s) designed with restriction enzyme sites, Linker sequence and His tag, and then transfected into E.coli DH5αafter linked with pMD18-T, respectively. The plasmids were extracted and restricted, and then linked via a hydrophilic and low-charge Linker sequence, which was then restricted simultaneously to construct the recombinant plasmid of pMD18-IL6-IFNα. This recombinant plasmid and expression vector pET32a were restricted and linked to construct the pET32-IL6-IFNα plasmid. The plasmid was transformed into E.coli DH5α and Rosetta (DE3), and then expressed under different concentrations' IPTG and times. The results showed that the pET32-IL6-IFNαwas constructed successfully. …. The results show that the expression plasmid is successfully constructed and IL6-IFNα protein is expressed in E.coli. This study will be a foundation for further study and application of IL6-IFNα as a novel efficient immunoadjuvant. |
3楼2012-10-05 09:51:33
