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hujiaoff

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[求助] GenBnak序列提交问题

最近提交了几个序列,回复要点如下:We have noticed that some or all of the sequences contain internal stop codons and/or reading frame shifts (insertions/deletions based on BLAST similarity search results and/or an alignment).
This generally indicates problems such as:
  - errors in the nucleotide sequence
  - sequences have not been trimmed of low quality
  - problems with feature annotation which may be due to:
    translation in the wrong reading frame or on the wrong strand
    translation does not have the correct exon spans
    translation includes noncoding sequence, such as introns or UTR's
序列都是双向测序,与Genbank已有的同种序列比对相似性为99%或100%,而且在填写注释时都是与已有的同种序列比对后确定的,不知为什么还会出现问题。由于是第一次接触这方面,现在不知道从哪方面入手。希望虫友不吝赐教!
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