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issaclai

木虫 (小有名气)

[求助] 求助:审稿意见中有些不明白的地方,请教各位了

我的论文中只写了培养基,每个方法里面写了细菌培养好后离心洗涤的处理过程。但审稿人提出:How are the cultures done? Pre culture or main culture.Centrifugation process?? How many bacterial cells were counted??
不知道该怎么回答,特别是里面的pre culture 和main culture是什么意思,请教各位了!
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issaclai

木虫 (小有名气)

引用回帖:
2楼: Originally posted by wjf008 at 2012-07-30 10:25:27
其实人家的意思是说,文章里面菌体的培养液是如何得到的?该菌体的培养液在整个实验过程里是种子培养还是发酵培养?人家还建议楼主要把培养液的菌体浓度写清楚。

能告诉我您的QQ号吗,我不是学生物的,所以很多都不懂,想具体请教您关于这句话的意思!

我做的是大肠杆菌,培养基是买的,然后配置的,
我的文章中是这么写的Escherichia coli was precultured in LB medium. The sterile culture medium contained 5 g NaCl, 10 g peptone ( purchased from sigma ), 5 g yeast extract ( purchased from sigma ) per liter. The pH of medium was adjusted to 7.0 before autoclaving. It was sterilized in high pressure steam for 30 min at 121 ℃.
3楼2012-07-30 11:53:35
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wjf008

金虫 (正式写手)


【答案】应助回帖

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感谢参与,应助指数 +1
rainwander: 金币+1, 鼓励交流 2012-07-30 18:18:50
issaclai: 金币+10 2012-08-04 08:03:03
其实人家的意思是说,文章里面菌体的培养液是如何得到的?该菌体的培养液在整个实验过程里是种子培养还是发酵培养?人家还建议楼主要把培养液的菌体浓度写清楚。
2楼2012-07-30 10:25:27
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issaclai

木虫 (小有名气)

我的文章中是这么写的:
Escherichia coli was precultured in LB medium. The sterile culture medium contained 5 g NaCl, 10 g peptone ( purchased from sigma ), 5 g yeast extract ( purchased from sigma ) per liter. The pH of medium was adjusted to 7.0 before autoclaving. It was sterilized in high pressure steam for 30 min at 121 ℃.
审稿意见是:
Culture medium: 1) It lacks details: How are the cultures done? Pre culture or main culture.Centrifugation process?? How many bacterial cells were counted?? Just mentioning LB medium is not sufficient.
4楼2012-07-30 11:55:18
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wjf008

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issaclai: 金币+10 2012-08-04 08:03:17
这个问题人家是说你对培养物的制备说的太不详细了。Culture medium: 1) It lacks details: How are the cultures done? Pre culture or main culture.Centrifugation process?? How many bacterial cells were counted?? Just mentioning LB medium is not sufficient.
主要是缺少细节:培养物的如何得到的?培养条件楼主没提到,是多少温度,多少时间等等。种子培养还是发酵培养。离心过程的条件,包括转速、时间等。细胞计数的方法要涉及到。
5楼2012-07-31 09:41:41
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