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替硝唑（tinidazole）2012年版英国药典标准
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caohouping
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痴夷子皮: 金币+2, 谢谢参与，欢迎常来哦。 2012-07-19 15:22:16
gaojingmei: 金币+25, ★★★很有帮助, O(∩_∩)O谢谢 2012-07-19 21:44:16
感谢参与，应助指数 +1
痴夷子皮: 金币+2, 谢谢参与，欢迎常来哦。 2012-07-19 15:22:16
gaojingmei: 金币+25, ★★★很有帮助, O(∩_∩)O谢谢 2012-07-19 21:44:16
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http://www.drugfuture.com/standard/search.aspx • British Pharmacopoeia Volume I & II • Monographs: Medicinal and Pharmaceutical Substances Tinidazole General Notices (Ph. Eur. monograph 1051) C8H13N3O4S 247.3 19387-91-8 Action and use Antiprotozoal; antibacterial. Ph Eur DEFINITION 1-[2-(Ethylsulfonyl)ethyl]-2-methyl-5-nitro-1H-imidazole. Content 98.0 per cent to 101.0 per cent (dried substance). CHARACTERS Appearance Almost white or pale yellow, crystalline powder. Solubility Practically insoluble in water, soluble in acetone and in methylene chloride, sparingly soluble in methanol. IDENTIFICATION First identification A, C. Second identification A, B, D, E. A. Melting point (2.2.14): 125 °C to 128 °C. B. Ultraviolet and visible absorption spectrophotometry (2.2.25). Test solution Dissolve 10.0 mg in methanol R and dilute to 100.0 mL with the same solvent. Dilute 1.0 mL of this solution to 10.0 mL with methanol R. Spectral range 220-350 nm. Absorption maximum At 310 nm. Specific absorbance at the absorption maximum 340 to 360. C. Infrared absorption spectrophotometry (2.2.24). Comparison tinidazole CRS. D. Thin-layer chromatography (2.2.27). Test solution Dissolve 20 mg of the substance to be examined in methanol R and dilute to 10 mL with the same solvent. Reference solution Dissolve 20 mg of tinidazole CRS in methanol R and dilute to 10 mL with the same solvent. Plate TLC silica gel GF254 plate R. Pretreatment Heat at 110 °C for 1 h and allow to cool. Mobile phase butanol R, ethyl acetate R (25:75 V/V). Application 10 µL. Development Over 2/3 of the plate. Drying In air. Detection Examine in ultraviolet light at 254 nm. Results The principal spot in the chromatogram obtained with the test solution is similar in position and size to the principal spot in the chromatogram obtained with the reference solution. E. To about 10 mg add about 10 mg of zinc powder R, 0.3 mL of hydrochloric acid R and 1 mL of water R. Heat in a water-bath for 5 min and cool. The solution gives the reaction of primary aromatic amines (2.3.1). TESTS Appearance of solution The solution is clear (2.2.1) and not more intensely coloured than reference solution Y5 (2.2.2, Method II). Dissolve 1.0 g in acetone R and dilute to 20 mL with the same solvent. Related substances Liquid chromatography (2.2.29). Protect solutions from light. Test solution Dissolve 10.0 mg of the substance to be examined in 10.0 mL of methanol R and dilute to 100.0 mL with the mobile phase. Reference solution (a) Dilute 1.0 mL of the test solution to 100.0 mL with the mobile phase. Dilute 1.0 mL of this solution to 10.0 mL with the mobile phase. Reference solution (b) Dissolve 5.0 mg of tinidazole impurity A CRS and 5.0 mg of tinidazole impurity B CRS in 10.0 mL of methanol R and dilute to 100.0 mL with the mobile phase. Dilute 2.0 mL of this solution to 10.0 mL with the mobile phase. Reference solution (c) Dilute 1.0 mL of reference solution (b) to 50.0 mL with the mobile phase. Column: • — size: l = 0.25 m, Ø = 3.0 mm; • — stationary phase: octylsilyl silica gel for chromatography R (5 µm). Regular column conditioning by subsequent flushing with 50 mL of water R, 100 mL of methanol R, 25 mL of water R and 100 mL of the mobile phase is recommended. Mobile phase acetonitrile R, methanol R, water R (10:20:70 V/V/V). Flow rate 0.5 mL/min. Detection Spectrophotometer at 320 nm. Injection 20 µL. Run time 1.5 times the retention time of tinidazole. Relative retention With reference to tinidazole (retention time = about 6 min): impurity A = about 0.6; impurity B = about 0.7. System suitability Reference solution (b): • — resolution: minimum 2.0 between the peaks due to impurities A and B. Limits: • — impurities A, B: for each impurity, not more than the area of the corresponding peak in the chromatogram obtained with reference solution (c) (0.2 per cent); • — unspecified impurities: for each impurity, not more than the area of the principal peak in the chromatogram obtained with reference solution (a) (0.10 per cent); • — total: not more than 4 times the area of the principal peak in the chromatogram obtained with reference solution (a) (0.4 per cent); • — disregard limit: 0.5 times the area of the principal peak in the chromatogram obtained with reference solution (a) (0.05 per cent). Heavy metals (2.4.8) Maximum 20 ppm. 1.0 g complies with test D. Prepare the reference solution using 2 mL of lead standard solution (10 ppm Pb) R. Loss on drying (2.2.32) Maximum 0.5 per cent, determined on 1.000 g by drying in an oven at 105 °C. Sulfated ash (2.4.14) Maximum 0.1 per cent, determined on 1.0 g. ASSAY Dissolve 0.150 g in 25 mL of anhydrous acetic acid R. Titrate with 0.1 M perchloric acid, determining the end-point potentiometrically (2.2.20). 1 mL of 0.1 M perchloric acid is equivalent to 24.73 mg of C8H13N3O4S. STORAGE Protected from light. IMPURITIES Specified impurities A, B. A. 2-methyl-5-nitro-1H-imidazole, B. 1-[2-(ethylsulfonyl)ethyl]-2-methyl-4-nitro-1H-imidazole. |
2楼2012-07-19 15:20:02
xhj0631
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gaojingmei: 金币+25, ★★★很有帮助, O(∩_∩)O谢谢 2012-07-19 21:44:28
感谢参与，应助指数 +1
gaojingmei: 金币+25, ★★★很有帮助, O(∩_∩)O谢谢 2012-07-19 21:44:28
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Tinidazole.doc http://kuai.xunlei.com/d/GRVPTRWWAXAG?p=130497 |
3楼2012-07-19 17:46:24
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