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lixiaojing1318
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[求助]
求高人翻译关于水稻转录组测序的一段摘要
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Understanding the dynamics of eukaryotic transcriptome is essential for studying the complexity of transcriptional regulation and its impact on phenotype. However, comprehensive studies of transcriptomes at single base resolution are rare, even for modern organisms, and lacking for rice. Here, we present the first transcriptome atlas for eight organs of cultivated rice. Using high-throughput paired-end RNA-seq, we unambiguously detected transcripts expressing at an extremely low level, as well as a substantial number of novel transcripts, exons, and untranslated regions. An analysis of alternative splicing in the rice transcriptome revealed that alternative cis-splicing occurred in ;33% of all rice genes. This is far more than previously reported. In addition, we also identified 234 putative chimeric transcripts that seem to be produced by transsplicing, indicating that transcript fusion events are more common than expected. In-depth analysis revealed a multitude of fusion transcripts that might be by-products of alternative splicing. Validation and chimeric transcript structural analysis provided evidence that some of these transcripts are likely to be functional in the cell. Taken together, our data provide extensive evidence that transcriptional regulation in rice is vastly more complex than previously believed. |
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yangxiuhuaya
铜虫 (小有名气)
- 翻译EPI: 8
- 应助: 0 (幼儿园)
- 金币: 175.7
- 帖子: 68
- 在线: 11.4小时
- 虫号: 1545573
- 注册: 2011-12-21
- 性别: GG
- 专业: 微生物生理与生物化学
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lixiaojing1318: 金币+30, 翻译EPI+1, ★★★很有帮助 2012-07-04 09:16:53
lixiaojing1318: 金币+30, 翻译EPI+1, ★★★很有帮助 2012-07-04 09:16:53
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了解真核转录的动态，对于研究转录调控的复杂性及其对表型的影响是非常有必要的。但是对于转录组在单碱基水平上的综合研究，哪怕是现代生物，目前也鲜有报导，而对于稻谷就更不用说了。在这篇文章中，我们首次公布了我们自己栽培的8种水稻转录组图谱。通过高通量末端配对RNA测序技术，我们准确无误地检测到了许多极低水平的转录表达，以及大量新的转录本，外显子和非编码区。水稻转录组选择性剪接分析显示，水稻全长基因有33%存在选择性顺式剪接。这个量比以往的报道要多得多。此外，我们还发现了234个转录本，可能是通过反式剪接产生的。这表明，转录本融合现象比我们预计的更常见。进一步分析显示，许多融合的转录本有可能是选择性剪接的副产物。转录本结构分析和验证表明，其中的某些转录本可能在细胞中承担某种功能。总之，我们的数据表明，水稻中的转录调控比我们以前所认为的要复杂得多。 好久没做基因了，有些专有名词记得不太清楚了，希望有所帮助，，，， |
2楼2012-07-03 16:48:59
