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lixiaojing1318

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Understanding the dynamics of eukaryotic transcriptome is essential for studying the complexity of transcriptional regulation
and its impact on phenotype. However, comprehensive studies of transcriptomes at single base resolution are rare,
even for modern organisms, and lacking for rice. Here, we present the first transcriptome atlas for eight organs of cultivated
rice. Using high-throughput paired-end RNA-seq, we unambiguously detected transcripts expressing at an extremely low
level, as well as a substantial number of novel transcripts, exons, and untranslated regions. An analysis of alternative
splicing in the rice transcriptome revealed that alternative cis-splicing occurred in ;33% of all rice genes. This is far more
than previously reported. In addition, we also identified 234 putative chimeric transcripts that seem to be produced by transsplicing,
indicating that transcript fusion events are more common than expected. In-depth analysis revealed a multitude of
fusion transcripts that might be by-products of alternative splicing. Validation and chimeric transcript structural analysis
provided evidence that some of these transcripts are likely to be functional in the cell. Taken together, our data provide
extensive evidence that transcriptional regulation in rice is vastly more complex than previously believed.
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yangxiuhuaya

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