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ncyxoy: ½ð±Ò+6, ¡ï¡ï¡ïºÜÓаïÖú, ллÄãµÄÓ¦Öú£¡ 2012-07-03 17:45:40
ncyxoy: ½ð±Ò+6, ¡ï¡ï¡ïºÜÓаïÖú, ллÄãµÄÓ¦Öú£¡ 2012-07-03 17:45:40
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Simultaneous quantification of polymethoxylated flavones and coumarins in Fructus aurantii and Fructus aurantii immaturus using HPLC-ESI-MS/MS ×÷Õß: Chen, HF (Chen, Hai-Fang)1; Zhang, WG (Zhang, Wu-Gang)2; Yuan, JB (Yuan, Jin-Bin)1; Li, YG (Li, Yan-Gang)1; Yang, SL (Yang, Shi-Lin)2; Yang, WL (Yang, Wu-Liang)1 À´Ô´³ö°æÎï: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS ¾í: 59 Ò³: 90-95 DOI: 10.1016/j.jpba.2011.10.013 ³ö°æÄê: FEB 5 2012 ±»ÒýƵ´Î: 0 (À´×Ô Web of Science) ÒýÓõIJο¼ÎÄÏ×: 18 [ ²é¿´ Related Records ] ÒýÖ¤¹ØÏµÍ¼ ÕªÒª: The major lipid-soluble constituents in Fructus aurantii (zhiqiao) and Fructus aurantii immaturus (zhishi) are polymethoxylated flavones (PMFs) and coumarins. In the present study, a high-performance liquid chromatography with electrospray ionization tandem mass spectrometry method was developed to quantify PMFs (nobiletin, tangeretin, 5-hydroxy-6,7,8,4'-tetramethoxyflavone, and natsudaidai) and coumarins (marmin, meranzin hydrate, and auraptene) simultaneously. PMFs and coumarins were detected by electrospray ionization tandem mass spectrometry in positive ion mode and quantified with multiple reaction monitor. Samples were separated on a Diamonsil C(18) (150 mm x 4.6 mm, 5 mu m) column using acetonitrile and formic acid-water solution as a mobile phase in gradient mode with a flow rate at 0.5 mL/min. All calibration curves showed good linearity (r(2) > 0.9977) within the test ranges. Variations of the intraday and interday precisions were less than 4.07%. The recoveries of the components were within the range of 95.79%-105.04% and the relative standard deviations were less than 3.82%. The method developed was validated with acceptable accuracy, precision, and extraction recoveries and can be applied for the identification and quantification of four PMFs and three coumarins in citrus herbs. (C) 2011 Elsevier B.V. All rights reserved. Èë²ØºÅ: WOS:000297716500012 ÎÄÏ×ÀàÐÍ: Article ÓïÖÖ: English ×÷Õ߹ؼü´Ê: Polymethoxylated flavones; Coumarin; Citrus; HPLC-ESI-MS/MS; Quantification KeyWords Plus: PERFORMANCE LIQUID-CHROMATOGRAPHY; OXYGEN HETEROCYCLIC-COMPOUNDS; CITRUS ESSENTIAL OILS; HYDROXYLATED POLYMETHOXYFLAVONES; MASS-SPECTROMETRY; CELL-LINES; IDENTIFICATION; NOBILETIN; MARMIN ͨѶ×÷ÕßµØÖ·: Yang, WL (ͨѶ×÷Õß),Jiangxi Univ Tradit Chinese Med, Key Lab Modern Preparat TCM, Minist Educ, Nanchang 330004, Jiangxi, Peoples R China µØÖ·: 1. Jiangxi Univ Tradit Chinese Med, Key Lab Modern Preparat TCM, Minist Educ, Nanchang 330004, Jiangxi, Peoples R China 2. Jiangxi Univ Tradit Chinese Med, Natl Pharmaceut Engn Ctr Solid Preparat Chinese H, Nanchang 330004, Jiangxi, Peoples R China µç×ÓÓʼþµØÖ·: yangwuliang@163.com »ù½ð×ÊÖúÖÂл: »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ Important National Science & Technology Specific Projects 2009ZX09103-350 NSFC 30660230 [ÏÔʾ»ù½ð×ÊÖúÐÅÏ¢][Òþ²Ø»ù½ð×ÊÖúÐÅÏ¢] This work was supported by Important National Science & Technology Specific Projects (2009ZX09103-350), NSFC (30660230). ³ö°æÉÌ: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS Web of Science ·ÖÀà: Chemistry, Analytical; Pharmacology & Pharmacy ѧ¿ÆÀà±ð: Chemistry; Pharmacology & Pharmacy IDS ºÅ: 857KD ISSN: 0731-7085 |

7Â¥2012-07-03 17:00:06














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