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FIG. 11 shows inhibitory activities of the wheat bran extract against the expression of the transcription factor NF-¦ÊB for cytokines. EXPERIMENTAL EXAMPLE 1-3:Assay for Inhibitory Activity against Production of Cytokine ¨C RT-PCR EXPERIMENTAL EXAMPLE 1-4-1: Inhibitory Activity against NF-¦ÊB Expression- Luciferase Assay FIG. 14 shows inhibitory activities of isolates from the wheat bran extract against the production of cytokines. As used herein, the term ¡°allergic diseases¡± means clinical symptoms that are caused by allergic reactions induced upon the activation of mast cells, such as the degranulation of mast cells. Prostaglandins promote the migration of leukocytes to inflammation sites while the inflammatory cytokines induce the expression of iNOS, which catalyzes the production of NO, causative of cytotoxicity and various inflammatory reactions, and the expression of COX-2, responsible for inflammation and pain. |
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FIG. 11 shows inhibitory activities of the wheat bran extract against the expression of the transcription factor NF-¦ÊB for cytokines.ͼ11 ÂóôïÌáÈ¡Îï¶Ôϸ°ûÒò×ÓµÄת¼Òò×ÓNF-kB±í´ïµÄÒÖÖÆ»îÐÔ EXPERIMENTAL EXAMPLE 1-3:Assay for Inhibitory Activity against Production of Cytokine ¨C RT-PCRÊÔÑéÀý1-3 ϸ°ûÒò×ÓÉú³ÉÒÖÖÆ»îÐÔµÄRT-PCR²â¶¨ EXPERIMENTAL EXAMPLE 1-4-1: Inhibitory Activity against NF-¦ÊB Expression- Luciferase AssayÊÔÑéÀý1-4-1£ºÓ«¹âËØÃ¸·¨²â¶¨NF-kB±í´ïÒÖÖÆ»îÐÔ FIG. 14 shows inhibitory activities of isolates from the wheat bran extract against the production of cytokines.ͼ14 ÂóôïÌáÈ¡Îï·ÖÀëµ¥Ìå¶Ôϸ°ûÒò×ÓÉú³ÉµÄÒÖÖÆ»îÐÔ As used herein, the term ¡°allergic diseases¡± means clinical symptoms that are caused by allergic reactions induced upon the activation of mast cells, such as the degranulation of mast cells. ÔÚÕâÀÊõÓï¡°±äÓ¦ÐÔ¼²²¡¡±ÊÇÖ¸ÓÉÖîÈç·Ê´óϸ°ûÍÑ¿ÅÁ£µÈ·Ê´óϸ°û»î»¯ËùÓÕ·¢µÄ±äÓ¦ÐÔ·´Ó¦ËùÖµÄÁÙ´²Ö¢×´¡£Prostaglandins promote the migration of leukocytes to inflammation sites while the inflammatory cytokines induce the expression of iNOS, which catalyzes the production of NO, causative of cytotoxicity and various inflammatory reactions, and the expression of COX-2, responsible for inflammation and pain. ǰÁÐÏÙËØ´Ù½ø°×ϸ°ûÏòÑ×Ö¢²¿Î»Ç¨ÒÆ£¬¶øÑ×֢ϸ°ûÒò×ÓÓÕµ¼ÓÕµ¼ÐÔÒ»Ñõ»¯µªºÏøµÄ±í´ï£¬ºóÕß´ß»¯NOµÄÉú³É£¬ÊÇϸ°û¶¾ÐԺ͸÷ÖÖÑ×Ö¢·´Ó¦µÄÔÒò£»iNOS»¹´ß»¯ÓëÑ×Ö¢ºÍÌÛÍ´Ïà¹ØµÄ»·Ñõ»¯Ã¸2µÄ±í´ï¡£ |
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