|
|
【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★
amisking: 金币+2, BioEPI+1, 鼓励发帖交流问题。 2012-06-11 20:27:59
jyalhc: 金币+8, ★★★★★最佳答案 2012-06-19 23:54:35
FN Thomson Reuters Web of Knowledge
VR 1.0
PT J
AU Jiang, Y
Ding, CB
Zhang, L
Yang, RW
Zhou, YH
Tang, L
AF Jiang, Yu
Ding, Chun-bang
Zhang, Li
Yang, Ruiwu
Zhou, Yonghong
Tang, Li
TI Identification of the genus Epimedium with DNA barcodes
SO JOURNAL OF MEDICINAL PLANTS RESEARCH
LA English
DT Article
DE Epimedium; DNA barcode; ITS; rbcL; matK; psbA-trnH
ID LAND PLANTS; SPECIES IDENTIFICATION; RBCL; SEQUENCES; MARKERS
AB The traditional identification for Epimedium is inadequate due to the morphological variation and complicated procedure. DNA barcoding is a new technique for species identification by a standard DNA region. However, there has been no consensus emerged for plant barcoding due to the low rates of evolution in plastid DNA. In this paper, we compared the performance of four candidate DNA regions (psbA-trnH, ITS, rbcL, matK) in ten species of Epimedium. To select a suitable barcode, ranking criteria included: the success efficiency of polymerase chain reaction (PCR) amplification and sequencing; genetic divergences; the barcoding gap. The psbA-trnH represented easy amplification, high level of interspecific divergence and well-separated barcoding gap. The other three loci (ITS, rbcL, matK) was inadequate for application. We concluded that the psbA-trnH region was the better potentially DNA barcode for the genus Epimedium.
C1 [Jiang, Yu; Ding, Chun-bang; Zhang, Li; Yang, Ruiwu; Tang, Li] Sichuan Agr Univ, Coll Biol & Sci, Yaan 625014, Peoples R China.
[Zhou, Yonghong] Sichuan Agr Univ, Key Lab Crop Genet Resources & Improvement, Minist Educ, Yaan 625014, Peoples R China.
RP Ding, CB (reprint author), Sichuan Agr Univ, Coll Biol & Sci, Yaan 625014, Peoples R China
EM dcb@sicau.edu.cn
NR 21
TC 0
Z9 0
PU ACADEMIC JOURNALS
PI VICTORIA ISLAND
PA P O BOX 5170-00200 NAIROBI, VICTORIA ISLAND, LAGOS 73023, NIGERIA
SN 1996-0875
J9 J MED PLANTS RES
JI J. Med. Plants Res.
PD NOV 30
PY 2011
VL 5
IS 28
BP 6413
EP 6417
DI 10.5897/JMPR11.545
PG 5
WC Chemistry, Medicinal
SC Pharmacology & Pharmacy
GA 873WE
UT WOS:000298913200004
ER
--------------------------------------------------------------------------------
EF |
|
回复此楼
点击这里搜索更多相关资源