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ÌâÄ¿£ºIdentification of the genus Epimedium with DNA
barcodes
ÆÚ¿¯Ãû£ºJournal of Medicinal Plants Research
¾í/Ò³£ºVol. 5(28), pp. 6413-6417, 30 November,  2011
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FN Thomson Reuters Web of Knowledge
VR 1.0
PT  J
AU  Jiang, Y
Ding, CB
Zhang, L
Yang, RW
Zhou, YH
Tang, L
AF  Jiang, Yu
Ding, Chun-bang
Zhang, Li
Yang, Ruiwu
Zhou, Yonghong
Tang, Li
TI  Identification of the genus Epimedium with DNA barcodes
SO  JOURNAL OF MEDICINAL PLANTS RESEARCH
LA  English
DT  Article
DE  Epimedium; DNA barcode; ITS; rbcL; matK; psbA-trnH
ID  LAND PLANTS; SPECIES IDENTIFICATION; RBCL; SEQUENCES; MARKERS
AB  The traditional identification for Epimedium is inadequate due to the morphological variation and complicated procedure. DNA barcoding is a new technique for species identification by a standard DNA region. However, there has been no consensus emerged for plant barcoding due to the low rates of evolution in plastid DNA. In this paper, we compared the performance of four candidate DNA regions (psbA-trnH, ITS, rbcL, matK) in ten species of Epimedium. To select a suitable barcode, ranking criteria included: the success efficiency of polymerase chain reaction (PCR) amplification and sequencing; genetic divergences; the barcoding gap. The psbA-trnH represented easy amplification, high level of interspecific divergence and well-separated barcoding gap. The other three loci (ITS, rbcL, matK) was inadequate for application. We concluded that the psbA-trnH region was the better potentially DNA barcode for the genus Epimedium.
C1  [Jiang, Yu; Ding, Chun-bang; Zhang, Li; Yang, Ruiwu; Tang, Li] Sichuan Agr Univ, Coll Biol & Sci, Yaan 625014, Peoples R China.
[Zhou, Yonghong] Sichuan Agr Univ, Key Lab Crop Genet Resources & Improvement, Minist Educ, Yaan 625014, Peoples R China.
RP  Ding, CB (reprint author), Sichuan Agr Univ, Coll Biol & Sci, Yaan 625014, Peoples R China
EM  dcb@sicau.edu.cn
NR  21
TC  0
Z9  0
PU  ACADEMIC JOURNALS
PI  VICTORIA ISLAND
PA  P O BOX 5170-00200 NAIROBI, VICTORIA ISLAND, LAGOS 73023, NIGERIA
SN  1996-0875
J9  J MED PLANTS RES
JI  J. Med. Plants Res.
PD  NOV 30
PY  2011
VL  5
IS  28
BP  6413
EP  6417
DI  10.5897/JMPR11.545
PG  5
WC  Chemistry, Medicinal
SC  Pharmacology & Pharmacy
GA  873WE
UT  WOS:000298913200004
ER  

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DOI: 10.5897/JMPR11.545
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