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谢婷1988

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[交流] 关于溴酚蓝染色机制已有2人参与

请问哪位知道溴酚蓝对蛋白质和DNA染色的机制，或者提供下参考书籍也可以，谢谢！

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小丹木木: 金币+2, 鼓励交流 2012-06-05 14:07:59

Bromophenol blue is also used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. Since bromophenol blue carries a slight negative charge at moderate pH, it will migrate in the same direction as DNA or protein in a gel; the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in TAE buffer or TBE buffer, bromophenol blue migrates at the same rate as a DNA fragment of approximately 500 base pairs. Xylene cyanol and Orange G may also be used for this purpose.