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【答案】应助回帖
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s771042285: 金币+30, 翻译EPI+1, ★★★★★最佳答案 2012-05-26 13:49:46
题目:ε-多聚赖氨酸产生菌种筛选
Screening of ε-polylysine Producing Strain
摘要：从多地采集土样作为初筛源，对ε-多聚赖氨酸产生菌的筛选体系进行了一系列的研究，并对筛选出来的菌种进行分离鉴定，拟分离出具有较高产量的ε-多聚赖氨酸产生菌。
Soil samples collected from varity of fields as the primary screen sources a series of study was conducted on the screening system of ε-polylysine producing strain. In order to isolate strains with high productivity of ε-polylysine the strains screened were isolated and identified.
对于采集回来的土样，进行了富集后再用于接种，会比不富集的更易筛得菌种。Compareed with the non-enriched soil samples inoculated with enriched ones strains were easier to obtain. 本实验采用的是SG培养基（液体）进行富集。In this experiment SG medium was used for enrichment.
用加入美蓝和重铬酸钾的SG培养基进行初筛，根据透明圈的大小初筛出产碱菌，复筛进行Dr试验和甲基橙试验，选出均为阳性反应的菌种。
SG medium with addition of methylene blue and potassium dichromate was applied to primary screen and alkali producing strains were selected according to the diameter of the transparent zone. Dr and methyl orange experiment were conducted in the second screening process and strains with positive reaction in both of the two experiments were selected.
复筛后对菌种进行摇瓶发酵试验，检测吸光度，用标准品做曲线，选得产量较高的几种菌。
After second screening fermentation experiment was conducted and absorbance was determined. According to the standard curve made by standard sunstance high productivity strains were obtained.
对复筛出来的菌进行一系列菌种形态特征、培养特征和生理生化特征，16SrDNA基因序列进行研究与分析。对照文献，对筛选出来的菌种进行分类，命名。
The strains selected were studied and annlysed through morphology, culture character and physiology biochemical character as well as 16SrDNA sequence. The screened strains were classified and named with the reference of doucuments.
关键词：ε-多聚赖氨酸；菌种筛选；菌种鉴定；发酵
Key words: ε-polylysine; Strain screening; Strain identification; Fermentation |
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