24小时热门版块排行榜

返回列表

蓝灰

金虫 (初入文坛)

应助: 0 (幼儿园)
金币: 1693.8
散金: 10
帖子: 42
在线: 58.8小时
虫号: 1135951
注册: 2010-10-31
性别: GG
专业: 食品科学基础

[求助] 豁出去了 100金币求助文献邮箱627607144@qq.com

Role of Mobile DNA in the Evolution of Vancomycin-Resistant Enterococcus faecalis

Prevalence and antibiotic resistance profiles of Enterococcus species in chicken at slaughter level; absence of vanA and vanB genes in E. faecalis and E. faecium

The relevance of gene transfer to the safety of food and feed derived from genetically modified (GM) plants

Enterococci as probiotics and their implications in food safety

Characterization of functional, safety, and probiotic properties of Enterococcus faecalis UGRA10, a new AS-48-producer strain

Enterococcus populations in artisanal Manchego cheese: Biodiversity, technological and safety aspects

Functional and Safety Aspects of Enterococci Isolated from Different Spanish Foods

Enterococci in foods—a conundrum for food safety

Safety assessment of dairy microorganisms: The Enterococcus genus

Safety and potential risks of enterococci isolated from traditional fermented capers

Safety aspects of enterococci from the medical point of view

Comparative analysis of genetic diversity and incidence of virulence factors and antibiotic resistance among enterococcal populations from raw fruit and vegetable foods, water and soil, and clinical samples

Risk factors in enterococci isolated from foods in Morocco: Determination of antimicrobial resistance and incidence of virulence traits

The Tyrosine Decarboxylation Test Does Not Differentiate Enterococcus faecalis from Enterococcus faecium

Regulating the Safety of Probiotics - The European Approach

Safety evaluation of probiotics

回复此楼

» 猜你喜欢

» 本主题相关价值贴推荐，对您同样有帮助:

哪有可以用的桥梁外文文献啊。求助！！知网的不能下载啊，有没其他渠道！已经有14人回复
organic Letters 投稿模板求助已经有12人回复
求毕业论文查重的软件,100金币已经有11人回复
第一篇SCI即将完工，求助参考文献怎么写进去？注：若最终发表，散尽金币谢诸位已经有10人回复
急求 materials letters A.F.W. Willoughby的邮箱已经有3人回复
链霉菌接合转移500金币求助已经有23人回复
100金币求助一个胺酯交换的反应已经有10人回复
辉瑞新上市的新药crizotinib的ALK酶抑制率是多少，如果有文献会追加100金币已经有8人回复
有关葡聚糖反应的问题，求助。。。撒金币已经有12人回复
求高人指点用matlab求解非线性方程组，解决了追加100金币；已经有11人回复
求助：大家一般到什么数据库下载外文文献呀？已经有6人回复
回答就有金币！求助PDDA！！已经有13人回复
【求助】50金币求助高手关于二茂铁电化学氧化还原测试的问题！已经有9人回复
100金币求助相关文献:分配系数和油水比(萃取相比)关系,急用,在线等. 已经有4人回复
【求助】二苯基硅二醇与环氧树脂的反应——100金币已经有22人回复
求助文献具体出处已经有7人回复

1楼 2012-05-20 21:37:02

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

huangfang15

铜虫 (初入文坛)

应助: 8 (幼儿园)
金币: 236.7
帖子: 43
在线: 7.2小时
虫号: 1803871
注册: 2012-05-08
专业: 食品科学基础

【答案】应助回帖

感谢参与，应助指数 +1

没明白你想要干嘛，这是一篇关于肠球菌的论文摘要吧？要关于这方面的文献？？？？

赞一下

回复此楼

2楼2012-05-22 10:36:33

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

huangfang15

铜虫 (初入文坛)

应助: 8 (幼儿园)
金币: 236.7
帖子: 43
在线: 7.2小时
虫号: 1803871
注册: 2012-05-08
专业: 食品科学基础

【答案】应助回帖

引用回帖:

2楼: Originally posted by huangfang15 at 2012-05-22 10:36:33:
没明白你想要干嘛，这是一篇关于肠球菌的论文摘要吧？要关于这方面的文献？？？？

Keywords:
antimicrobial resistance;enterococcus;insertion sequence;mobile genetic elements;plasmid;review;transposon

Abstract
Clin Microbiol Infect 2010; 16: 541–554

Abstract
Mobile genetic elements (MGEs) including plasmids and transposons are pivotal in the dissemination and persistence of antimicrobial resistance in Enterococcus faecalis and Enterococcus faecium. Enterococcal MGEs have also been shown to be able to transfer resistance determinants to more pathogenic bacteria such as Staphylococcus aureus. Despite their importance, we have a limited knowledge about the prevalence, distribution and genetic content of specific MGEs in enterococcal populations. Molecular epidemiological studies of enterococcal MGEs have been hampered by the lack of standardized molecular typing methods and relevant genome information. This review focuses on recent developments in the detection of MGEs and their contribution to the spread of antimicrobial resistance in clinically relevant enterococci.

Introduction
Enterococci are important nosocomial pathogens [1]. They are uniquely armed for the antibiotic era and express intrinsic reduced susceptibility to major classes of antimicrobial agents and biocides [2,3]. The high propensity of enterococci to acquire and express new resistance determinants further enhances their ability to sustain antibiotic selection, promoting gastrointestinal colonization and nosocomial infections by antibiotic-resistant enterococci [4].

Transferable antimicrobial resistance in enterococci was first described in the early 1970s [5–7]. The detection and molecular clarification of transferable high-level vancomycin resistance in Enterococcus faecium in the late 1980s have further fuelled our interest in the mechanisms and routes to antimicrobial resistance in enterococci [8–11]. Importantly, as residents of human and animal bowel flora, enterococci are in a position to acquire resistance genes from other commensals, which may subsequently proceed to other more pathogenic bacteria [12,13].

Molecular biological studies have elucidated complex functional properties of important mobile genetics elements (MGEs) involved in transferable resistance in enterococci [10,14,15]. However, there is a considerable gap in our knowledge on the molecular epidemiology of MGEs, their genetic content and composition, as well as their relative contributions to the spread of defined antimicrobial resistances. Recent progress in molecular typing methods and enterococcal genome information has provided new tools and necessary insights for filling this gap. This review focuses on MGEs involved in the spread and expression of clinically important antimicrobial resistance in enterococci and their potential contribution to the spread of hospital-adapted clonal lineages of E. faecium and Enterococcus faecalis.

Plasmids as Important Vehicles for Genetic Information in Enterococci
By definition, plasmids are semi-autonomously replicating extrachromosomal genetic elements. Differences in replication strategies and modular structures profoundly affect plasmid properties, such as size, copy number, host dependence and host range [16]. The essential backbones for successful plasmids include genetic modules encoding self-replication, stable inheritance and the ability to transfer between bacteria. Accessory plasmid content is integrated in between functional plasmid backbone modules and represents a huge reservoir of genetic variability, often with unknown functions, that is shared among different bacterial genera through horizontal gene transfer.

There are several criteria to classify plasmids in general and plasmids related to Gram-postive bacteria in particular. The mode of replication has been used to distinguish rolling circle replication plasmids and theta-replicating plasmids. In addition, plasmids that fail to co-reside in the same cell are grouped in incompatibility (Inc) groups [17,18]. Inc18 plasmids constitute a large group of enterococcal/streptococcal plasmids with a broad host range [19,20]. Pheromone-responsive plasmids represent a unique group of self-transferable (conjugative) narrow host range plasmids mostly described in E. faecalis [21]. pAD1, pAM373 and pCF10 are well-known examples of pheromone-responsive plasmids, where the conjugative process is initiated as a response to short peptide pheromones produced by pheromone-responsive plasmid-free recipient strains mediating intercellular aggregation and high-frequency DNA transfer. Recently, Weaver et al. [22] proposed a new family (RepA_N) of broadly distributed plasmids in Gram-positive bacteria encompassing pheromone-responsive plasmids of E. faecalis, as well as pRUM of E. faecium. Detailed sequence comparisons of the replication initiator protein suggest that the replicons have evolved along with their specific host, explaining their relatively narrow host range.

Plasmid replicon modules (replicons) have recently been used as targets of more simplistic methods for typing and epidemiological tracing of plasmids conferring antimicrobial resistance (R-plasmids). Other essential gene sets for plasmid survival, such as mobilization regions, have also been suggested as targets [23]. Given the modular evolution and genetic plasticity of plasmids, it is of note that schemes based on different core elements may not be congruent [16]. Carattoli et al. [18] developed a PCR-based plasmid typing method based on the replication regions from various plasmid incompatibility groups occurring in Enterobacteriaceae. A similar approach was recently described for the detection of plasmids from enterococci and other Gram-positive bacteria [24]. On the basis of 111 published sequences from Gram-positive bacteria, 19 replicon families (rep-family) and several unique replicons were identified. Using this PCR-based typing system, pCF10 (rep9)-, Inc18 (rep1 and rep2)- and pUSA02 (rep7)-related replicons were identified as being most prevalent in E. faecalis strains (n = 28), whereas Inc18 (rep2)-, pRI1 (rep14)- and pRUM (rep17)-related replicons dominated in E. faecium strains (n = 51) of human and animal origin [24]. However, approximately 30% of the strains tested did not support any rep-detection, indicating the presence of unidentified rep-types.

The enterococcal-specific parts of the rep-detection system described by Jensen et al. [24] have been used by others. A recent study of an epidemiologically diverse collection of E. faecium strains (n = 93) revealed a high prevalence of Inc18 (rep2)-, pRUM (rep17)- and pHTβ (repunique)-related replicons [25]. The actual enterococcal typing scheme accounted for approximately 60% of the total number of plasmids visualized by S1-nuclease analyses. Interestingly, strains belonging to hospital-adapted clades (CC17-related) yielded a significant higher number of rep types and pRUM (rep17)-related replicons in particular, indicating a role in accessory plasmid DNA for promoting hospital adaptation. Rep-typing of extended E. faecalis strain collections has so far shown a dominance of pheromone-responsive plasmid (rep8 and 9)-, pS86 (rep6)- and Inc18 (rep1 and 2)-related replicons (J. Sun, S. Xiaobo, T. Mikalsen, J. U. Ericson Sollid, A. Sundsfjord, unpublished observations). Other comprehensive studies include vancomycin-resistant E. faecium and E. faecalis strains causing hospital outbreaks in five continents, from 1986 to date (A. R. Freitas, M. V. Francia, L. Peixe, C. Novais, L. B. Jensen, R. J. Willems, F. Baquero, T. M. Coque, unpublished observations). Among E. faecium, mostly CC17-related, a high diversity of rep types could be identified; small [pB82 (rep11), pRI1 (rep14), pEF418 (rep18), pCIZ2 (repunique)] or medium to large plasmids [Inc18 (rep1 and 2), pRUM (rep17), pHTβ (repunique)], with vanA linked to Inc18- and pRUM-like plasmids in most cases. Vancomycin-resistant E. faecalis isolates belonging to major clonal complexes (CC2, CC9 and CC87) contained a lower diversity of plasmids, which were mostly associated with the narrow host pheromone-responsive pAD1 (rep9) and Inc18-type (rep1 and 2) plasmids.

Linkage of clinically important resistance determinants to specific replicon types in enterococci is of interest for predicting potential transfer to other bacterial genera by conjugative broad host range plasmids. The application of pulsed-field gel electrophoresis of S1-nuclease-digested enterococcal DNA has proved very useful for the identification and sizing of enterococcal plasmids because they appear as linearized bands (5–400 kb) on a faint genomic background [25–27]. Physical linkage between defined plasmid rep types and resistance determinants can be visualized by co-hybridization analysis of linearized plasmid DNAs [25]. Co-hybridization analysis and plasmid sequencing have shown linkage of the vanA operon to Inc18-, pHTbeta- and pRUM-related plasmids in E. faecium [25,28–31].

Plasmid genomic analysis in enterococci has been hindered by the comparably high number of extrachromosomal elements in many (especially clinical) strains; the tremendous size of several of these plasmids and the multicopy insertion sequence (IS) elements located on them complicating raw data and contig assembly. Sequencing of large multiresistance megaplasmids (>200 kb) are in progress, revealing the presence of multiple resistance determinants linked to known virulence-associated proteins (J. A. Laverde-Gómez, G. Werner, unpublished observations).

赞一下

回复此楼

3楼2012-05-22 10:44:58

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖