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【答案】应助回帖
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lina8804: 金币+20, 翻译EPI+1, ★★★很有帮助 2012-04-27 12:18:02
3.15 The study of way DNA interact with BCDO
Above experiments indicate that the system that BCDO determined DNA belongs to a quenching form system, according to the Stern-Volmer (S-V) equation: F0 / F = 1 + Kq [Q] (in the equation : F0 and F stand for fluorescence intensity of system without and with quenching agent DNA ,respectively.[Q] is the concentration of quenching agent DNA, Kq is quenching constant, its unit is L mol-1, and its size reflects the strength of affinity between fluorescence probe and DNA molecular , figure 3.31 fitting by F0 / F ~ CDNA figure, the results as shown in figure 3.32.
AS the Figure 3.32 shows , F0 / F - CDNA has a good linear relationship, after fitted,the equation of F0 / F - CDNA is: F0 / F = 1.05688 + 0.05808 CDNA, in the equation: CDNA's unit is the Mg L-1, the F0 / F - CDNA equation of DNA agree with static quenching equation, the slope for the linear is the quenching constant Kq, due to the concentration unit of DNA in the above fitted equation is Mg L-1, according to the Kq value of above equation for unit conversion, the Kq equels 9.12 x 106 L, mol-1, this illustrated fully that in this paper a self-made new type fluorescent molecules such as BCDO had interacted strongly with DNA , and had a very strong affinity between them.
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