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qwj616

新虫 (正式写手)

[求助] 求助-如何得到羟基自由基(.OH)、单线态氧分子(1O2)、超氧负离子(O2-)

请大家帮忙,非常感激!!!如何得到羟基自由基(.OH)、单线态氧分子(1O2)、超氧负离子(O2-),我做实验需要做这几种粒子的对比实验,说明单线态氧分子、羟基自由基、超氧负离子没有类似的实验现象,但是我不知道该如何获取这些试剂或者原料,然后再将其应用在试验中,请大家帮忙,非常感激!!!
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王丕

木虫 (小有名气)

【答案】应助回帖

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qwj616: 金币+10, ★★★★★最佳答案 2012-06-01 08:47:33
These content could be find in the supporting information of "Chem. Commun., 2011, 47, 9468–9470".
The concentration of the stock H2O2 solution was titrated according to iodometry.
1O2 was generated by the reaction of H2O2 with NaClO. O2 .- was created by the
enzymatic reaction of xanthine/xanthine oxidase (XA/XO) at 25 °C for 5 min. The
experiments were performed under anaerobic conditions.[8] Deionized water was
degassed with Ar for 20 min. NO was generated from SNP. SNP was added into
degassed deionized water under Ar atmosphere then stirred for 30 min at room
temperature. The probe solution was also degassed before the reaction with NO.
ROO· was generated by AAPH. ·OH was generated by Fenton reaction (Fe2+ +
H2O2→ Fe3+ + ·OH + OH−.[9]
7楼2012-05-03 17:14:18
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