24小时热门版块排行榜    

查看: 532  |  回复: 1
本帖产生 1 个 翻译EPI ,点击这里进行查看

xeti

金虫 (正式写手)

[求助] 微生物方面几句话

下面几句话比较难懂,请求各位帮忙。谢谢! The purified OmcS antibodies were tested for specificity using Western blot analysis (One-Step Western complete kit with tetramethyl benzidine [TMB]; GenScript) of total proteins prepared from cell lysates of the G. sulfurreducens wild type and the mutant strain  in which the gene encoding OmcS had been deleted. Total proteins (10 g) were separated by 10% SDS-PAGE, blotted onto membranes using a semidry transfer cell (Bio-Rad), and incubated with the purified OmcS antibodies. Only one band with a molecular mass corresponding to that of the OmcS protein (i.e., 50 kDa) was detected with Western blot analysis in the wild-type cell lysate, whereas no bands were detected in the cell lysate
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

频回眄睐

至尊木虫 (职业作家)

【答案】应助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★
xeti: 金币+10, 翻译EPI+1, ★★★很有帮助, 非常感谢!顺便问下英语是如何达到那么高水平的 2012-04-20 12:17:38
我们用特异性印迹(一步法西方完整套件四甲基联苯胺 [TMB] ;)分析提纯Omcs 的总蛋白细胞G蛋白质组。在这些全蛋白中野生型及突变株的 OmcS 基因已被删除。通过10% SDS-PAGE和Bio-Rad转移,以及提纯的OcmS抗体,我们分离了10g全蛋白.在野生类型的细胞中,只有一个与OmcS蛋白质分子量相似的分子(例如,50KDa)可以用特异性印迹分析检测,其它的则不能。
相逢,相识,相知
2楼2012-04-20 12:05:28
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 xeti 的主题更新
信息提示
请填处理意见