| ²é¿´: 537 | »Ø¸´: 1 | ||
| ±¾Ìû²úÉú 1 ¸ö ·ÒëEPI £¬µã»÷ÕâÀï½øÐв鿴 | ||
xeti½ð³æ (ÕýʽдÊÖ)
|
[ÇóÖú]
΢ÉúÎï·½Ãæ¼¸¾ä»°
|
|
| ÏÂÃæ¼¸¾ä»°±È½ÏÄѶ®£¬ÇëÇó¸÷λ°ï档лл£¡ The purified OmcS antibodies were tested for specificity using Western blot analysis (One-Step Western complete kit with tetramethyl benzidine [TMB]; GenScript) of total proteins prepared from cell lysates of the G. sulfurreducens wild type and the mutant strain in which the gene encoding OmcS had been deleted. Total proteins (10 g) were separated by 10% SDS-PAGE, blotted onto membranes using a semidry transfer cell (Bio-Rad), and incubated with the purified OmcS antibodies. Only one band with a molecular mass corresponding to that of the OmcS protein (i.e., 50 kDa) was detected with Western blot analysis in the wild-type cell lysate, whereas no bands were detected in the cell lysate |
» ²ÂÄãϲ»¶
ÉúÎïѧѧ˶Çóµ÷¼Á£º351·ÖÒ»Ö¾Ô¸ÄϾ©Ê¦·¶´óѧÉúÎïѧרҵ
ÒѾÓÐ5È˻ظ´
Çóµ÷¼Á£¬Ò»Ö¾Ô¸ÏÃÃÅ´óѧ£¬ÉúÎïÓëÒ½Ò©£¬×Ü·Ö272£¬±¾¿Æ211
ÒѾÓÐ5È˻ظ´
µ÷¼Á
ÒѾÓÐ9È˻ظ´
070300»¯Ñ§Ñ§Ë¶311·ÖÇóµ÷¼Á
ÒѾÓÐ11È˻ظ´
Ò»Ö¾Ô¸±±½»´ó²ÄÁϹ¤³Ì×Ü·Ö358Çóµ÷¼Á
ÒѾÓÐ7È˻ظ´
308Çóµ÷¼Á
ÒѾÓÐ12È˻ظ´
0703»¯Ñ§
ÒѾÓÐ18È˻ظ´
ÉúÎïѧ308·ÖÇóµ÷¼Á£¨Ò»Ö¾Ô¸»ª¶«Ê¦´ó£©
ÒѾÓÐ5È˻ظ´
292Çóµ÷¼Á
ÒѾÓÐ4È˻ظ´
286Çóµ÷¼Á
ÒѾÓÐ10È˻ظ´
Ƶ»Øíííù
ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)
- ·ÒëEPI: 110
- Ó¦Öú: 13 (СѧÉú)
- ¹ó±ö: 0.566
- ½ð±Ò: 17171.4
- É¢½ð: 1586
- ºì»¨: 30
- ɳ·¢: 3
- Ìû×Ó: 3338
- ÔÚÏß: 545.1Сʱ
- ³æºÅ: 1238300
- ×¢²á: 2011-03-19
- ÐÔ±ð: GG
- רҵ: ½ðÊôÓлú»¯Ñ§
¡¾´ð°¸¡¿Ó¦Öú»ØÌû
¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï
xeti: ½ð±Ò+10, ·ÒëEPI+1, ¡ï¡ï¡ïºÜÓаïÖú, ·Ç³£¸Ðл£¡Ë³±ãÎÊÏÂÓ¢ÓïÊÇÈçºÎ´ïµ½ÄÇô¸ßˮƽµÄ 2012-04-20 12:17:38
xeti: ½ð±Ò+10, ·ÒëEPI+1, ¡ï¡ï¡ïºÜÓаïÖú, ·Ç³£¸Ðл£¡Ë³±ãÎÊÏÂÓ¢ÓïÊÇÈçºÎ´ïµ½ÄÇô¸ßˮƽµÄ 2012-04-20 12:17:38
| ÎÒÃÇÓÃÌØÒìÐÔÓ¡¼££¨Ò»²½·¨Î÷·½ÍêÕûÌ×¼þËļ׻ùÁª±½°· [TMB] £»£©·ÖÎöÌá´¿Omcs µÄ×ܵ°°×ϸ°ûGµ°°×ÖÊ×é¡£ÔÚÕâЩȫµ°°×ÖÐÒ°ÉúÐͼ°Í»±äÖêµÄ OmcS »ùÒòÒѱ»É¾³ý¡£Í¨¹ý10% SDS-PAGEºÍBio-Rad×ªÒÆ£¬ÒÔ¼°Ìá´¿µÄOcmS¿¹Ì壬ÎÒÃÇ·ÖÀëÁË10gÈ«µ°°×.ÔÚÒ°ÉúÀàÐ͵Äϸ°ûÖУ¬Ö»ÓÐÒ»¸öÓëOmcSµ°°×ÖÊ·Ö×ÓÁ¿ÏàËÆµÄ·Ö×Ó£¨ÀýÈ磬50KDa£©¿ÉÒÔÓÃÌØÒìÐÔÓ¡¼£·ÖÎö¼ì²â£¬ÆäËüµÄÔò²»ÄÜ¡£ |

2Â¥2012-04-20 12:05:28














»Ø¸´´ËÂ¥