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Enzymatic browning causes color changes in fruits and vegetables, which result from the action of a group of enzymes called polyphenol oxidases. The effect of
irradiation on the PPO activity of fresh-cut celery is shown in Fig. 1. The PPO activity of irradiated celery was about 100 U, lower than that of nonirradiated celery
in 3 days of storage at 4 C, which indicated that the PPO activity of fresh-cut celery was significantly inhibited by irradiation. The PPO activity of irradiated celery, however, increased solely with further increasing storage time at 4 C, and the PPO activities of fresh-cut celery irradiated with 1.0 and 1.5 kGy were lower than
that of nonirradiated and irradiated ones with 0.5 kGy up to 9 days of storage. The results suggest that the quality of fresh-cut celery could be maintained and the
shelf-life of the celery would be extended through controlling the enzymatic browning by irradiation. Enzymatic browning may be controlled by using both
physical and chemical methods, and in most cases both are employed. Physical methods may include reduction of temperature and/or oxygen, use of modified atmosphere packaging or edible coating, or treatment with gamma irradiation or high pressure (Garcia & Barrett,2002).

   The respiration rate of irradiated fresh-cut celery was measured (Fig. 2). It was obvious that the respiration rate after 9 days of storage at 4 C was inhibited by irradiation treatment in 0.5, 1.0, and 1.5 kGy and the respiration rate decreased with increasing irradiation dose. Since senescence is highly related to the respiration, high respiration rate would lead to tissue breakdown of freshcut celery. The lowered respiration by 1.0 and 1.5 kGy delayed the senescence of fresh-cut celery and reduced the loss of nutritional substances. Therefore, it concluded that the irradiation was able to increase shelf-life of fresh-cut celery and to decrease the loss of nutritional value in fresh-cut celery by lowering the respiration rate.
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