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mike_308

银虫 (小有名气)

[求助] 求助用罗丹明B异硫氰酸酯(36877-69-7)标记牛血清蛋白的详细方法

如题~用罗丹明B异硫氰酸酯标记牛血清蛋白是否和用FITC是一样的?
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mike_308

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引用回帖:
6楼: Originally posted by xhjggl at 2012-04-23 10:38:47:
把786-058编号输入,点击搜索,就可以下载到PDF文档

我查询36877-69-7,但是没说明书?
虽然觉得和FITC应该没什么区别,但因为是用这个写材料,所以感觉还是慎重点好
8楼2012-04-28 19:23:19
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xhjggl

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【答案】应助回帖

★ ★ ★
感谢参与,应助指数 +1
zhaohq1209: 金币+3, 鼓励应助~欢迎交流~thanks 2012-04-29 09:44:26
HOOK™ Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine)
蛋白罗丹明标记试剂盒       
Description:
(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides.(5/6) TAMRA absorbs green visible light at 546nm and emits an orange-red visible light at a maximum emission of 575nm.The NHS ester group provides the simplest and most commonly used group for labeling proteins. The succinimidyl ester group reacts with primary amines in lysine side chains and N-terminal amines forming a stable, covalent amide bond.This kit utilizes Tube-O-REACTOR™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands-on time and effort. The (5/6) TAMRA-SE labeling reagent is also available in our single use OneQuant™ format.
Features:
Dye preweighed and supplied in single use  vials (Also available separately);Suitable for most proteins;Utilizes  single Tube Reactor for minimum hands on time and no protein loss.application for Labeling of proteins, peptides and nucleic acids with a red fluorescent dye.;Suitable for antibody labeling.Store: -15~-20℃
编号        包装        价格        产地
786-058        5 Reactions        2,709        GBios
2楼2012-04-17 13:57:40
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mike_308

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引用回帖:
2楼: Originally posted by xhjggl at 2012-04-17 13:57:40:
HOOK™ Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine)
蛋白罗丹明标记试剂盒       
Description:
(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on te ...

有详细一些的使用说明吗?
3楼2012-04-20 12:11:10
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xhjggl

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【答案】应助回帖

有,可以到上海生工网站上下载说明书看看

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4楼2012-04-20 13:22:11
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