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EB对BCDO-DNA体系荧光强度的影响 为了进一步考察本组自制的荧光化合物BCDO与DNA的作用方式，在BCDO-DNA体系中加入不同量的EB，扫描荧光光谱，结果如图3.34所示。 由图3.34可知，EB对BCDO-DNA体系的发射峰位置没有影响，但是在发射波长517.8nm处发射峰的荧光强度随EB浓度的增加而下降，且在589nm处出现了EB-DNA体系的荧光发射峰，其峰强度随着EB浓度的增加而增大。加入EB使得BCDO-DNA体系荧光强度降低，说明EB分子可能取代了BCDO-DNA体系中部分BCDO分子，形成了EB-DNA复合体系。EB能使BCDO-DNA体系在517.8nm处的荧光产生猝灭现象，而且猝灭程度随EB浓度的增加而加大，据此推断，BCDO和DNA之间的主要作用方式应该与EB和DNA的作用方式相似，均属于嵌插方式。 |
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至尊木虫 (职业作家)
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爱与雨下: 金币+2 2012-04-18 19:22:57
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EB has an effect on the fluorescence intensity of the BCDO-DNA system . For further investigation about our Group’s BCDO fluorescent compounds with DNA , we added a different amount of EB in the BCDO-DNA system , scanning fluorescence spectroscopy ,and the results were shown in Figure 3.34. From the figure 3.34 ,we known that EB has no effect on BCDO-DNA system of emission peak position, however in the emission wavelength fluorescence intensity of 517.8nm emission peaks decline when the EB concentrations increase , and appear at 589nm fluorescence emission peaks of the EB-DNA system increased with the increase in the concentration of its peak strength as the EB. We added EB into the BCDO-DNA system ,then the fluorescence intensity reduced, indicating that EB may replace the BCDO-DNA part of BCDO molecules in the system, forming a EB-DNA complex system. EB can make BCDO-DNA system in 517.8nm by fluorescence quenching and quenching was based on concentrations of EB .According to the phenomenon ,we concluded that between BCDO and DNA's primary role should be similar to the EB and the role of DNA, belonging to the insert method . |
2楼2012-04-18 18:13:44