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lina8804: 金币+15, 翻译EPI+1, ★★★很有帮助 2012-04-09 09:53:04

The results of above experiments show that the homemade BCDO compound with a larger conjugate structure, has good molecular planarity, and can be inserted into DNA base, thereby causing hyperchromic effect and hypochromic effect of ultraviolet spectroscopy. When the electronic transfer occurs between BCDO and vernine G, it causes energy transfer with no radiation, thereby causing the quenching of fluorescence spectrum. Because of the electrophilic effect of carbonyl, the carbon atom of BCDO has a certain positive electricity, and has electrostatic effect with anionic phosphoric acid skeleton of DNA, so that BCDO and DNA react by embeding pratly and static electricity. The method has the advantages of stable system for determining DNA, and satisfactory results. The linear range is 0 ~ 6.0 mg • L-1, and the detection limit is 1.03 mg • L-1.

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