原句是 Cells were harvested by centrifugation at 6000 ×g at 4 °C for 5 min,
resuspended in 50 mM Tris–HCl, pH 7.5, and subjected to a shearing
force for 2 min at a low setting in a commercially available blender。
其中 6000 ×g at 4 °C 不明白是什么意思,请求大神们帮忙下,最好帮忙把这句话完整翻一下,谢了!!