Peptides were purified by HPLC [ApexPresil C18 8 m column (Jones Chromatography, Lakewood, CO）using a linear gradient from 0 to 50% acetonitriley/H2O(0.1%trif luoroacetic acid) over 40-min elution at 47% acetonitrile].
The peptide primary structure was confirmed by electrospray
ionization MS 文献里面是这样纯化合成的多肽的 请问各位大虾流动相梯度是怎么调整的 从小到大么 恩 over40min elution at 47的乙腈是什么意思 是说在这个浓度下冲洗47分钟 分离得最开吗？
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