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È«ÎÄ£ºJasmonate and related signalling compounds have a crucial role in both host immunity and development in plants, but the
molecular details of the signalling mechanism are poorly understood. Here we identify members of the jasmonate
ZIM-domain (JAZ) protein family as key regulators of jasmonate signalling. JAZ1 protein acts to repress transcription of
jasmonate-responsive genes. Jasmonate treatment causes JAZ1 degradation and this degradation is dependent on activities
of the SCFCOI1 ubiquitin ligase and the 26S proteasome. Furthermore, the jasmonoyl¨Cisoleucine (JA¨CIle) conjugate, but not
other jasmonate-derivatives such as jasmonate, 12-oxo-phytodienoic acid, or methyl-jasmonate, promotes physical
interaction between COI1 and JAZ1 proteins in the absence of other plant proteins. Our results suggest a model in which
jasmonate ligands promote the binding of the SCFCOI1 ubiquitin ligase to and subsequent degradation of the JAZ1 repressor
protein, and implicate the SCFCOI1¨CJAZ1 protein complex as a site of perception of the plant hormone JA¨CIle.




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Furthermore, the jasmonoyl¨Cisoleucine (JA¨CIle) conjugate, but not
other jasmonate-derivatives such as jasmonate, 12-oxo-phytodienoic acid, or methyl-jasmonate, promotes physical
interaction between COI1 and JAZ1 proteins in the absence of other plant proteins. Our results suggest a model in which
jasmonate ligands promote the binding of the SCFCOI1 ubiquitin ligase to and subsequent degradation of the JAZ1 repressor
protein, and implicate the SCFCOI1¨CJAZ1 protein complex as a site of perception of the plant hormone JA¨CIle.
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°®ÓëÓêÏÂ: ½ð±Ò+1 2012-03-22 13:05:12
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