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[求助]
麻烦哪位高手帮忙翻译一下，后半段实在是搞不懂，我急着做PPT的，谢谢了。
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麻烦哪位高手帮忙翻译一下，后半段实在是搞不懂，我急着做PPT的，谢谢了。 全文：Jasmonate and related signalling compounds have a crucial role in both host immunity and development in plants, but the molecular details of the signalling mechanism are poorly understood. Here we identify members of the jasmonate ZIM-domain (JAZ) protein family as key regulators of jasmonate signalling. JAZ1 protein acts to repress transcription of jasmonate-responsive genes. Jasmonate treatment causes JAZ1 degradation and this degradation is dependent on activities of the SCFCOI1 ubiquitin ligase and the 26S proteasome. Furthermore, the jasmonoyl–isoleucine (JA–Ile) conjugate, but not other jasmonate-derivatives such as jasmonate, 12-oxo-phytodienoic acid, or methyl-jasmonate, promotes physical interaction between COI1 and JAZ1 proteins in the absence of other plant proteins. Our results suggest a model in which jasmonate ligands promote the binding of the SCFCOI1 ubiquitin ligase to and subsequent degradation of the JAZ1 repressor protein, and implicate the SCFCOI1–JAZ1 protein complex as a site of perception of the plant hormone JA–Ile. 翻译： Furthermore, the jasmonoyl–isoleucine (JA–Ile) conjugate, but not other jasmonate-derivatives such as jasmonate, 12-oxo-phytodienoic acid, or methyl-jasmonate, promotes physical interaction between COI1 and JAZ1 proteins in the absence of other plant proteins. Our results suggest a model in which jasmonate ligands promote the binding of the SCFCOI1 ubiquitin ligase to and subsequent degradation of the JAZ1 repressor protein, and implicate the SCFCOI1–JAZ1 protein complex as a site of perception of the plant hormone JA–Ile. |
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生化道长
至尊木虫 (知名作家)
- 翻译EPI: 49
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爱与雨下: 金币+1 2012-03-22 13:05:12
ficz: 金币+25, 翻译EPI+1, ★★★★★最佳答案, 感谢道长。 2012-03-23 20:54:09
爱与雨下: 金币+1 2012-03-22 13:05:12
ficz: 金币+25, 翻译EPI+1, ★★★★★最佳答案, 感谢道长。 2012-03-23 20:54:09
| 另外，在缺少其他植物蛋白的情况下，JA-Ile偶联物而不是其他茉莉酸衍生物诸如茉莉酸、12 - 羰基-植二烯酸或者甲基-茉莉酸等促进COI1和JAZ1蛋白之间的物理作用。我们的结果提出了一个模型，在这个模型中茉莉酸配体促进SCFCOI1泛素连接酶的结合，随后发生JAZ1阻遏物蛋白的降解，并且说明SCFCOI1-JAZ1蛋白复合体是植物激素JA-Ile的一个感知位点。 |
2楼2012-03-22 11:09:02